用核分析技术研究锌在克汀鼠脑亚细胞和蛋白中的分布

Nuclear analysis techniques were combined with cellular and general approaches of fundamental research in biology. Animal model of iodine-deficient rats was induced by feeding diets with different iodine levels. The elements contents in various tissues were measured. The effect of iodine on other elements was discussed. Our study demonstrated the marked alterations in the spatial distribution of zinc and calcium of iodine-deficient rats brain during brain development stages. The contents of most elements in hippocampus were higher/equal than those in cerebral cortex and thalamus. Zn content in the hippocampal fissure was 1.5 times higher than that in other part of hippocampus. Comparison with control rats, both the content of iodine and the total iodine in thyroid of iodine-deficient rats were significantly low and the goiter was fund; in the iodine-deficient rats supplemented with adequate iodine, there are no any significant difference in terms of the content of iodine, the total iodine and weight of thyroid; in the deficient-iodine rats supplemented with excessive iodine the goiter was fund as well, but the total iodine was not different; in the iodine-deficient rats maximum binding capacity (MBC) of nuclear T3 receptor and T45' deiodinase were marked high, in the deficient-iodine rats supplemented with excessive iodine there was no difference in terms of MBC, but T45' deiodinase was significantly low. There results will be helpful to elucidate the mechanism of the effect of excessive-iodine intake on iodine-deficient population.

依据碘硒在甲状腺功能中的作用，及锌指结构的T3核受体的研究进展。本项目拟建立超微量微区生物样品的同步辐射X荧光定量分析方法，并与放射性标记、生化分离技术相岷希鄄炜送∈蟛钩涞夂臀螅吭诳送∈竽匝窍赴偷鞍追肿又械姆植急浠云诙约鬃聪俟δ芎腿钡獠〗懈钊肓私猓剿魑⒘吭氐纳镒饔谩?.