基于分子间多重作用力的协同样品前处理技术研究

Sample preparation is still considered the bottleneck of the whole analytical process. Its relative research has been widely concerned. Molecularly imprinted sample preparation technique has the characteristics of specific selectivity and good capability of resisting disturbances. So it has been widely used for trace analysis in complex samples. But there is still a problem of that the intermolecular force is single during recognition process. This research integrates molecularly imprinted technique and solid phase microextraction technique as well as monolithic material synthesis technology to develop synergistic sample preparation system based on multiple interactions between molecules. It is then coupled with high performance liquid chromatography for high selective and sensitive analysis of trace analytes in complex samples. The synergistic effects between inclusion capability of cyclodextrin itself and specific recognition ability of molecularly imprinted polymer will be studied in depth. The recognition mechanism of cyclodextrin molecularly imprinted polymer will also be clarified. This project will propose a novel synergistic sample preparation technique. The recognition system based on multiple intermolecular forces used in complex sample preparation will be further studied. Synergistic effects in the process of cyclodextrin molecularly imprinted polymers recognition will also be clarified. This study will promote the development of high selective molecularly imprinted sample preparation techniques.

样品前处理环节被视为整个分析过程的瓶颈，其研究备受关注。基于分子印迹的样品前处理技术具有特异选择性和抗基体干扰的特点，广泛应用于复杂样品中的痕量分析，但仍存在识别作用力单一的问题。本研究集合分子印迹技术、固相微萃取技术和整体材料制备技术，发展基于分子间多重作用力识别的协同样品前处理体系，联用高效液相色谱实现复杂样品中痕量分析物的高选择性分离和高灵敏检测，并深入研究基于环糊精功能单体的分子印迹固相微萃取整体材料在分子识别过程中本身固有的包结能力和分子印迹聚合物的特异性识别能力的协同效应，阐明环糊精分子印迹聚合物的识别机理。本研究提出协同样品前处理技术，深入研究基于多重作用力的分子识别体系在复杂样品前处理中的应用，阐明环糊精分子印迹聚合物在分子识别过程中的协同效应，推动高选择性分子印迹样品前处理技术的发展。

样品前处理环节被视为整个分析过程的瓶颈，其研究备受关注。基于分子印迹的样品前处理技术具有特异选择性和抗基体干扰的特点，广泛应用于复杂样品中的痕量分析，但仍存在识别作用力单一的问题。本研究集合分子印迹技术、固相微萃取技术和整体材料制备技术，发展基于分子间多重作用力识别的协同样品前处理体系。设计并合成了一系列的beta-环糊精（beta-CD）功能单体，分别进行了红外光谱、质谱、核磁谱表征，在此基础上分别制备了对硝基酚、双酚A和三氯生分子印迹固相微萃取涂层材料，其印迹因子分别为2.26、3.20和2.03，进一步研究了基于beta-环糊精功能单体分子印迹微萃取涂层的结构性能和萃取性能，应用于复杂实际样品中硝基酚类化合物、双酚类化合物、多氯酚类化合物的高效分离富集，联用高效液相色谱实现了高灵敏度和高选择性分析，且基于beta-环糊精功能单体分子印迹识别材料在分子识别过程中存在协同识别效应。环糊精空腔本身固有的包结能力和分子印迹聚合物的特异性识别能力能协同提高对目标分析物的识别能力。本研究提出的协同样品前处理技术，强化了多重作用力在分子识别过程中的作用，为发展新型样品前处理技术提供了新的思路。