新型多烯大环内酯抗生素农抗702诱导水稻抗瘟性及其诱抗机理研究

Agricultural antibiotic 702(ag-antibiotic 702) is a new polyene macrolide antibiotic which was discovered and identified by our group. Although spraying ag-antibiotic 702 can significantly improve rice resistance to blast disease at the seedling phase, it is not clear how to induce rice resistance to blast disease and its mechanism by ag-antibiotic 702. In our proposal, we will use ag-antibiotic 702 as an inducer and rice as research material to investigate ag-antibiotic 702 concentration of induction of resistance against rice blast disease, the induced resistance at the different stage of rice growth, the effect duration of rice blast resistance, and the defense assay by induction of resistance against different rice pathogen, which will provide the basic research for the application of ag-antibiotic702. Moreover, the effect of ag-antibiotic702 on membrane lipid peroxidation in rice, the phenolic metabolisn in rice leaves, the activity of protein related to the pathogenicity in rice leaves, and the antimicrobial active compounds produced by rice will be investigated to elucidate the induced resistance mechanism of rice blast disease by ag-antibiotic 702. Our results will be paved the foundation for further develop a new agricltural antibiotic for plant protection with the independent intellectual property right, which will be of most significance for ag-antibiotic702 application and protection of good quality but susceptible rice varieties.

农抗702是本项目组发现和鉴定的一种新型的多烯类大环内酯抗生素。在水稻苗期喷施农抗702药液后能对水稻瘟病菌产生抗瘟性,诱导水稻抗瘟性及其诱抗机理并不清楚。本项目以农抗702和水稻分别为诱导因子和研究作用对象,分别通过对水稻诱导抗瘟作用的农抗702作用浓度的筛选、对水稻不同生长发育时期的诱抗作用、对水稻诱抗作用的持续作用时间和诱抗作用对水稻不同病原菌的防御性测定等进行较深入研究,探索农抗702对水稻抗瘟性,为农抗702在实践中的应用提供科学依据;然后通过农抗702诱导水稻抗瘟性分别与水稻膜脂过氧化和活性氧清除系统的影响、与水稻叶片中酚类物质代谢的影响、与水稻叶片病程相关蛋白活性的影响和与产生相应抗菌活性物质等进行较深入研究,探索农抗702对水稻的诱抗机理,为进一步研发我国具有自主知识产权的新型植病杀菌剂奠定较坚实理论基础。对于农抗702的应用和保护品质好但易感病的水稻品种具有特别重要的意义

1）本项目以农抗 702和水稻分别为试验材料和研究作用对象，一是通过对农抗 702对水稻诱导抗瘟性作用浓度的筛选、对水稻不同生长发育时期的诱导抗瘟性作用、对水稻诱导抗瘟性作用的持续作用时间和诱导抗瘟性作用对水稻不同病原菌的防御性测定，探究农抗702诱导水稻抗瘟性，二是通过农抗702 诱导水稻抗瘟性分别对水稻膜脂过氧化和活性氧清除系统的影响、对水稻叶片中酚类物质代谢的影响、对水稻叶片病程相关蛋白活性的影响和对水稻产生的相应抗菌活性物质，探讨农抗702诱导水稻抗瘟机理进行研究。.农抗702能够显著提高水稻对稻瘟病的抗性，并对水稻抗稻瘟病的相关代谢有着积极的诱导作用。农抗702能够诱导过敏性反应及氧化酶活性的增强，增加水稻抗病相关酚类物质及其代谢酶的活性，提高水稻病程相关蛋白酶的活性，及诱导β-谷甾醇、豆甾醇、γ-豆甾醇、亚油酸甲酯、2,4-二叔丁基苯酚、亚麻酸，肉豆蔻酸、十一（碳）烷和正十六碳酸等抗菌活性物质的积累等抗病相关的生理防御机制来阻止稻瘟病菌对水稻的感染，侵入和蔓延，增强水稻对稻瘟病的抗性。从生理及代谢水平揭示了农抗720诱导水稻抗瘟性的机理，在农抗702诱导水稻抗稻瘟病提供了强有力的证据，并为进一步研发我国具有自主知识产权的新型植病杀菌剂，无论是从理论研究和实践应用上均有重要意义。.2）本项目发表了研究论文9篇,其中,国际英文学术期刊4篇,国内核心期刊5篇。.3）在本项目资助下,完成与本项目研究内容相关的14篇学位论文,其中硕士论文1篇,学士论文13篇。