天然产物itol A对褐飞虱的作用机理研究

The complexity of the skeleton and abundant functional groups of natural products given unique biological activities, and it has irreplaceable unique properties as the lead compound of pesticides. The previous research showed that itol A exhibited contact toxic activity against N. lugens. Therefore, the further research will clear to the other action mode of itol A against N. lugens, and the impact of enzyme and neurotransmitter to N. lugens. Comparative proteomics analysis and transcriptome of itol A to N. lugens would study. The objective of this study was to make a comprehensive utilization of itol A as a new insecticide for controlling the brown planthopper. This project also would give the guiding significance on the creation of new pesticides that have independent intellectual property rights.

天然产物骨架的复杂性和丰富的官能团化赋予了天然产物独特的生物活性，因而天然产物作为农药创制的先导化合物具有无法替代的独特性质。本项目将在前期首次明确天然产物itol A对褐飞虱长翅型成虫具有触杀活性的基础上，运用昆虫毒理学、分子生物学、蛋白组学等方法研究itol A对褐飞虱的作用机理。明确itol A对褐飞虱的作用方式、对褐飞虱的离子通道以及相关靶标酶系及神经递质的影响，并以蛋白质组学方法研究褐飞虱对itol A响应机制。项目将明确天然产物Itol A对褐飞虱的作用靶标位点，为该化合物作为农药先导结构提供研究基础。

项目明确了itol A对褐飞虱具有触杀、胃毒、拒食等作用方式，明确了该化合物对褐飞虱的剂量及时间效应关系，itol A对褐飞虱长翅型成虫具有较强的触杀作用，其24H的LD50值为0.58μg/头；itolA不作用于AChE，但显著影响Na+/K+-ATPase、Ca2+-ATPase及Ca2+/Mg2+-ATPase的活性，通过影响褐飞虱的MFO、GST、SOD、CAT、ACP、GPX等解毒及氧化酶系而使昆虫死亡。该研究基本明确天然产物Itol A对褐飞虱的作用靶标位点，为该化合物作为农药先导结构提供研究基础。