玉米皮低聚糖阿魏酸酯对肠道艰难梭菌的抑制机制研究

Our previous research showed that, at genus level, feruloylated oligosaccharides significantly increased the abundance of Lactobacilli, while significantly decreased the abundance of Clostridium in the colon of rats. However, the regulatory mechanism is still unclear. In this research, Lactobacillus reuteri and Clostridium difficile were selected to investigate the inhibitory mechanism of Clostridium by infusion of feruloylated oligosaccharides. (1) The direct inhibition effect of feruloylated oligosaccharides on Clostridium difficile will be evaluated by the bacteriostasis in vitro. (2) Mixed culture of Lactobacillus reuteri and Clostridium difficile will be applied to investigate the inhibitory effect of Lactobacillus reuteri on Clostridium difficile with or without addition of feruloylated oligosaccharides. (3) Fermented products from Lactobacillus reuteri with or without addition of feruloylated oligosaccharides will be used to investigate the inhibitory effect of Lactobacillus reuterion Clostridium difficile; the substances exhibiting significant inhibition on Clostridium difficile will be identified. This work aims to fully elucidate the inhibition mechanism of feruloylated oligosaccharides on Clostridium difficile through these researches.

我们前期研究发现，玉米皮低聚糖阿魏酸酯（Feruloylated Oligosaccharides, FOs）在抑制肠道梭菌的同时，能够显著促进肠道乳酸菌生长，而其调节机制尚不清楚。本项目拟选择乳酸菌属代表性微生物罗伊氏乳杆菌及梭菌属代表性微生物艰难梭菌，从FOs直接影响梭菌及影响两种菌间互作关系的角度，研究低聚糖阿魏酸酯对肠道梭菌的抑制机制。主要研究内容为：（1）采用体外抑菌实验，研究FOs是否直接抑制艰难梭菌的生长。（2）将罗伊氏乳杆菌和艰难梭菌混合培养，研究FOs是否通过促进罗伊氏乳杆菌的生长而抑制艰难梭菌的生长。（3）分离添加与不添加FOs培养后的罗伊氏乳杆菌的发酵液，观察其对艰难梭菌的抑制效果；在此基础上，表征出对艰难梭菌具有显著抑制作用的物质。通过以上研究，阐明FOs对肠道艰难梭菌的抑制机制。

我们前期研究表明，低聚糖阿魏酸酯（Feruloylated Oligosaccharides, FOs）在抑制肠道梭菌的同时，能够显著促进肠道乳酸菌生长，具有显著缓解肠道炎症的功能，而其调节机制尚不清楚。本项目拟选择乳酸菌属代表性微生物罗伊氏乳杆菌及梭菌属代表性微生物艰难梭菌，从FOs直接影响梭菌及影响两种菌间互作关系的角度，研究低聚糖阿魏酸酯对肠道梭菌的抑制机制。主要研究内容为：（1）采用体外抑菌实验，研究FOs是否直接抑制艰难梭菌的生长。（2）将罗伊氏乳杆菌和艰难梭菌混合培养，研究FOs是否通过促进罗伊氏乳杆菌的生长而抑制艰难梭菌的生长。（3）分析添加与不添加FOs培养后的罗伊氏乳杆菌和艰难梭菌的发酵液。通过以上研究，阐明FOs对肠道艰难梭菌的抑制机制。