19F/1H双模磁共振分子成像研究

Molecular magnetic resonance imaging (MRI) enables non-invasive visualization of molecular levels in tissues in vivo, and will become an important imaging method for early diagnose of diseases. In this project, targeting the early diagnosis of ovarian cancer, a serial of amphiphilic peptide dendrons will be synthesized and used to construct stable nanoparticles combining with perflourocarbon via self-assemble progress. Modified with PEG and functional peptides,the specific flurione probes will enable tageting ovarian cancer cells noninvasively.The high sensitive 19F/1H MRI will be realized by using an open-designed transmit coil with a new geometric structure that can generate a high homogeneous B1 field. The decoupling effect of the phased array coils will be removed by using a matrix decoupling method, and auto-tuning of the coil to the dual-mode will be realzied by programming control.Since 19-flurione MRI bears no interference and aritifact from 1H necleus, the 19F/1H dual-mode MRI is especially suitable for both anatomic structure imaging and molecular imaging. The generation method of the imaging probes and the dual-mode imaging technique in this proposal hold great promise for future translational applications from benchside to bedside.

磁共振分子影像是应用影像学技术显示活体状态下组织、细胞和亚细胞水平的特定分子变化情况，是疾病早期诊断的重要手段。本项目以卵巢癌早期诊断为目标，应用双亲性树枝状大分子交联体构成的两亲性聚合物，以自组装方法包裹全氟化碳，并结合 PEG和靶向卵巢癌短肽对氟纳米粒子进行修饰，构建高稳定、高靶向性磁共振氟纳米探针；应用开放式发射线圈结构、窄带单频相控阵线圈、数字去耦以及程控自动调谐，实现高灵敏 19F/1H 双模磁共振成像。本项目实施有望获得一类具有我国自主知识产权、拥有潜在临床价值的MR 氟成像探针及相应的磁共振成像技术。本项目研究的氟纳米探针以及高灵敏 19F/1H 双模磁共振成像方法具有很强的实用性以及重要的科学意义和应用前景。

磁共振分子影像是应用影像学技术显示活体状态下组织、细胞和亚细胞水平的特定分子变化情况，是疾病早期诊断的重要手段。在本项目中，我们构建了一类新型磁共振纳米探针平台，开发了“智能”双模T2及19F纳米探针及磁共振T1-T2双模磁共振探针，有望用于肿瘤的磁共振诊断；同时，研发了相应的磁共振线圈系统及优化了磁共振成像方法。本项目研究的纳米探针及磁共振成像方法具有很强的实用性以及重要的科学意义和应用前景。本项目的实施为发展具有临床应用价值的高性能MRI分子探针和成像技术奠定了基础，促进了临床医学分子诊断的发展。