以模拟月壤为载体的生物膜厌氧生物制氮气基础研究

In recent 10 years, the research on lunar regolith simulant and the protection and guarantee of life support and safety of astranauts have been encouraged and highly supported bu NASA in USA.In this proposal,the lunar regolith simulant is uased to form the biofilm to achieve ananerobic ammonium oxidation (anammox) process in an upflow anaerobic sludge blanket (UASB) bioreactor, which can produce nitrogen gas from urine with addtion of proper ammount of ammonium or nitrate salts..The biofilm will be started up and acclimatized in an UASB bioreactor.The combined mechanisms, such as bionitrogen removal and bioproduction of nitrogen gas with the biofilm formed on the lunar regolith simulant and the characteristics of the biofilm, are planned to be investigated. The stoichiometry and stoichiometric relations and models of anammox with denitrification will be studied. The optimal process parameters and affective factors are going to be considered. The molecular biology of anammox and denitrifying microorganisms in the mixed sludge will be studied by extraction and purification of total genomic DNA from sludge samples, the sequence clones will be analyzed and blasted in GENEBANK to eastablish the family tree of microorganisms..This proposal is a preparing study plan for the option of technologies of simulant air production mixed with nitrogen gas for the future possible application in Lunar Base construction.

针对美国NASA近10年来高度重视模拟月壤与航天员生命保障技术研究的热点，以模拟月壤为载体材料，对配制的含有大量COD、NH4+、NO3－及尿液的模拟废水，用上流式厌氧污泥床（UASB）生物反应器进行启动和驯化形成生物膜；开展模拟月壤生物脱氮与制氮气过程的协同作用机理、生物膜特性及其影响因素研究；获取厌氧氨氧化（Anammox）、反硝化协同作用下的反应计量系数与计量关系模型，明确协同作用机理；研究有机环境下的Anammox过程及其与反硝化生物脱氮协同作用的优化工艺与各种影响因素；通过混溶物16 S rDNA扩增、电泳、克隆与基因测序，深入研究Anammox与反硝化微生物菌群的分子生物学，获得系统发育树。为将来月球基地建设、模拟空气制取等开展预先研究，准备技术基础。

针对美国NASA近10年来高度重视模拟月壤与航天员生命保障技术研究的热点，以模拟月壤为载体材.料，对配制的含有大量COD、NH4+、NO3－及尿液的模拟废水，用上流式厌氧污泥床（UASB）生物反应.器进行启动和驯化形成生物膜；开展模拟月壤生物脱氮与制氮气过程的协同作用机理、生物膜特性及.其影响因素研究；获取厌氧氨氧化（Anammox）、反硝化协同作用下的反应计量系数与计量关系模.型，明确协同作用机理；研究有机环境下的Anammox过程及其与反硝化生物脱氮协同作用的优化工艺.与各种影响因素；通过混溶物16 S rDNA扩增、电泳、克隆与基因测序，深入研究Anammox与反硝化微.生物菌群的分子生物学，获得系统发育树。为将来月球基地建设、模拟空气制取等开展预先研究，准.备技术基础。