表达鸡贫血病毒免疫原性蛋白的重组火鸡疱疹病毒的研究

基本信息
批准号:39970562
项目类别:面上项目
资助金额:12.00
负责人:赵晓岩
学科分类:
依托单位:中国农业科学院哈尔滨兽医研究所
批准年份:1999
结题年份:2002
起止时间:2000-01-01 - 2002-12-31
项目状态: 已结题
项目参与者:刘长军,王笑梅,王端,高志强,代春铭
关键词:
重组火鸡疱疹病毒免疫原性蛋白贫血病毒
结项摘要

Chicken infectious anemia (CIA) is caused by Chicken infectious anemia virus (CIAV), which causes destruction of erythroblastoid cells in the bone marrow and lymphoid depletion of the thymic cortex in susceptible chicks. CIAV can transmit vertically through the eggs of infected breeder flocks resulting in a severe anemia and trophy of the lymphoid organs. Also the virus can be transmitted horizontally when maternal antibody was disappeared, resulting in a sub-clinical infection that adversely affects growth and profitability in broiler flicks. CIAV has recently been noticed and considered to be one of the most important avian diseases. However, the methods used to control CIA now are not effective..In this study, we have amplified the genes of vp1 and vp2 of CIAV by PCR, and inserted them into genome DNA of HVT(Herpesvirus of Turkey) to construct recombinant HVT, which can express these two types of proteins. Also, we have made the research in the expression and immunogenicity of vp1 and vp2 of the recombinant virus. Our research contents completed include as follow:1.Sequencing and analyzing of encoding sequences of CIAV M9905 strain.2.Constructing 3 recombinant plasmid pCISIvp1-vp2,pCIvp1,pSIvp2 as DNA vaccine and comparing the protection of them in SPF chickens with inactive vaccine CUX-1.3 Expression of VP1 and VP2 genes of CIAV by baculorvirus expression systems4.Immunogenicity determination of VP1 and VP2 proteins of CIAV.5..Construction of a turkey herpesvirus recombinant containing VP1 and VP2 genes of CIAV.6.Immunogenicity determination of a turkey herpesvirus recombinant containing VP1 and VP2 genes of CIAV. Study on this project is laying a foundation of the research and development of effective CIA vaccine.

鸡传染性贫血病是鸡最重要的免疫抑制病之一,目前还没有安全有效的疫苗对该病进行预防。本研究拟采用PCR方法扩增和克隆鸡贫血病毒的VP1和VP2基因,并将其插入火鸡疱疹病毒℉VT)DNA中,构建表达这两种蛋白的重组HVT;同时对VP1和VP2在重组病毒中的表达及其免疫原性进行研究。为安全有效的鸡传染性贫血病疫苗的研制和开发奠定基础。

项目摘要

项目成果
{{index+1}}

{{i.achievement_title}}

{{i.achievement_title}}

DOI:{{i.doi}}
发表时间:{{i.publish_year}}

暂无此项成果

数据更新时间:2023-05-31

其他相关文献

1

病毒性脑炎患儿脑电图、神经功能、免疫功能及相关因子水平检测与意义

病毒性脑炎患儿脑电图、神经功能、免疫功能及相关因子水平检测与意义

DOI:10.13210/j.cnki.jhmu.20190508.001
发表时间:2019
2

中药对阿尔茨海默病β - 淀粉样蛋白抑制作用的实验研究进展

中药对阿尔茨海默病β - 淀粉样蛋白抑制作用的实验研究进展

DOI:10.3969/j.issn.1008-0805.2021.05.57
发表时间:2021
3

神经退行性疾病发病机制的研究进展

神经退行性疾病发病机制的研究进展

DOI:
发表时间:2018
4

组蛋白去乙酰化酶在变应性鼻炎鼻黏膜上皮中的表达研究

组蛋白去乙酰化酶在变应性鼻炎鼻黏膜上皮中的表达研究

DOI:10.16066/j.1672-7002.2021.06.013
发表时间:2021
5

SUMO特异性蛋白酶3通过调控巨噬细胞极化促进磷酸钙诱导的小鼠腹主动脉瘤形成

SUMO特异性蛋白酶3通过调控巨噬细胞极化促进磷酸钙诱导的小鼠腹主动脉瘤形成

DOI:10.3969/j.issn.1000-4718.2020.05.001
发表时间:2020

赵晓岩的其他基金

批准号:11105163
批准年份:2011
资助金额:30.00
项目类别:青年科学基金项目

相似国自然基金

1

鸡马立克氏病毒MDV和火鸡疱疹病毒HVT核酶杂交的研究

批准号:39170575
批准年份:1991
负责人:徐宜为
学科分类:C1802
资助金额:3.30
项目类别:面上项目
2

火鸡疱疹病毒基因组非必需片段的筛选

批准号:39370521
批准年份:1993
负责人:徐宜为
学科分类:C1802
资助金额:5.00
项目类别:面上项目
3

择瘤繁殖并表达绿色荧光蛋白的重组单纯疱疹病毒检测循环肿瘤细胞

批准号:81472013
批准年份:2014
负责人:张文
学科分类:H2603
资助金额:72.00
项目类别:面上项目
4

鸡贫血病毒的细胞嗜性研究

批准号:39570553
批准年份:1995
负责人:徐彦波
学科分类:C1801
资助金额:9.50
项目类别:面上项目