枸杞品质对根际微生物多样性的响应机制研究

The quality of Lycium barbarum L. is not only influenced by environmental factors, but also may be closely related to the structure of microbial population in the rhizosphere of Lycium Barbarum L.. In view of the different quality of Lycium Barbarum L. among the different producing area, the study use the HPLC method to determine main active ingredients of the ‘Ningqi-1’ Lycium Barbarum L. from Ningxia, Qinghai and Xinjiang, at the same time, rhizosphere microorganisms of the 5 regions were detected by high throughput sequencing. Useing professional software to analyze the datas, get the population diversity and differences of the rhizosphere microorganisms, find microbial population which effect the main active ingredients of Lycium barbarum L., finally reveal the biological factors which effect the quality of Lycium Barbarum L. fruit. The research of this project provide theoretical basis for improving the quality and protecting the origin of Lycium barbarum L., and keeping sustainable development of genuine Lycium barbarum L..

枸杞品质不仅受环境因子的影响,还可能与不同地区枸杞根际土壤微生物种群结构密切相关。本项目针对各产地枸杞品质存在差异性的问题， 应用HPLC等方法测定宁夏、青海、新疆‘宁杞1号’枸杞有效成分含量的同时，用高通量测序的方法对5个产区枸杞根际微生物进行分析，运用专业软件对研究数据进行统计分析，获得5个产区的根际微生物的种群多样性及种群差异，找到影响枸杞主要有效成分转化合成的微生物种群，从生物因子水平揭示根际微生物对枸杞品质形成的影响。本项目的研究为优质枸杞的生产、枸杞原产地保护、道地枸杞可持续发展等方面提供一定理论基础。

枸杞品质不仅受环境因子的影响,还可能与不同地区枸杞根际土壤微生物种群结构密切相关。本项目针对不同产地枸杞品质存在差异性的问题，测定宁夏、青海、新疆三个典型产区‘宁杞1号’枸杞有效成分含量的同时，用高通量测序的方法对不同产区枸杞根际微生物多样性进行分析，运用专业软件对数据进行统计分析，获得3个产区的根际微生物的种群多样性及种群差异，找到影响枸杞主要有效成分转化合成的微生物种群，从生物因子水平揭示根际微生物对枸杞品质形成的影响。本项目的研究为优质枸杞的生产、枸杞原产地保护、道地枸杞可持续发展等方面提供一定理论基础。