鼻腔M细胞体外模型的建立及其在鼻腔给药研究中的应用

M cells (microfold cells) play an important role in nasal drug delivery, which are the main absorption sites of macromolecules and particles. However, up to date, there is no in vitro nasal M cell model for in vitro evaluation of nasal administrated drugs. It was reported that co-culture of Caco-2 cells and Raji cells may transform the epithelial cells to M-like cells (intestinal M cells). Therefore, we try to transform Calu-3 cells to nasal M-like cells through co-culture of Calu-3 cells (similar to Caco-2 cells in features) and Raji cells, in order to study the transport and mechanism of action of drugs, especially macromolecules and particles, after nasal administration. This study may provide a stable in vitro platform for nasal administration, which may be helpful to further development of nano-scale formulations in nasal administration.

M细胞（微褶皱细胞）在鼻腔药物递送方面具有重要作用，是大分子药物及微粒的主要吸收部位。但至今仍无可用的鼻腔M细胞体外模型用于鼻腔给药的体外评价。文献报道Caco-2人结直肠腺癌细胞与Raji人Burkitt's淋巴瘤细胞共培养可诱导Caco-2细胞转化为具有M细胞特性的细胞（肠道M细胞）。因此，我们尝试通过Calu-3人肺腺癌细胞（与Caco-2细胞的特性类似）与Raji细胞的共培养，诱导Calu-3细胞转化为具有鼻腔M细胞特性的细胞，用于评价药物尤其是大分子药物和纳米药物在鼻腔给药后的转运情况及作用机理。此项研究将为鼻腔给药提供可靠的体外研究平台，有助于推动纳米递药系统在鼻腔给药方面的深入发展，具有明显的创新性。