巯基点击化学制备新型纳米复合物用于植物激素高敏快速生物传感研究

The thiols and thiolated chemicals are highly concerned in surface chemistry and synthetic chemistry. The self-assembling adsorption of various thiols on some metal electrode surfaces (e.g. Au) has been widely employed to fabricate various functional chemically modified electrodes, and many molecular bioprobes with thiol tags have been synthesized for metal nanomaterials-based bioanalysis. The thiol-based click chemistry refers to the addition of a S-H bond across a double or triple bond by either a free radical or ionic mechanism. Recently, the thiol-based click chemistry has already received extensive attention owing to its outstanding application potential in the fields of synthetic chemistry, material preparation and chemo-/biosensing, however, the thiolated polymers and their nanocomposites as enrichment materials for bioanalysis applications through the thiol-based click chemistry have not been reported to date. There is academic significance and reference value on the innovation and optimization of materials and methods for biosensing through the further study of the various new thiolated composite materials (containing graphene, carbon nanotubes, nano-/Au, Pt, Pd, etc.), utilization of the high reactivity of mercapto group in the thiol-based click chemistry which effective improvement of immobilization of the biological recognition molecules for signal output and amplification. In this work, we mainly study on the preparation and characterization of new nanocomposite through the thiol-based click chemistry, as well as the fabrication of novel biosensor for highly sensitive and rapaid detection of phytohormone.

硫醇和巯基化生物探针已广泛地用于电极表面修饰和化学/生物传感，但采用绿色合成法巯基点击化学制备的巯基化高分子复合材料的生物传感应用目前在文献中鲜见报导。深入研究各种巯基化的新型复合材料（含石墨烯、碳纳米管，纳米金、铂、钯等），利用巯基点击化学中巯基的高反应性赋予复合材料多功能化以提高其富集效率及高效固定生物识别分子，从而赋予生物传感界面放大信号/输出定量信号的功能，创新和优化相关生物传感材料和方法，具有新颖性、学术意义和应用参考价值。故此，我们提出本项目，主要研究：（1）基于巯基点击化学的新型纳米复合物的制备和表征；（2）构建新型生物传感器实现植物激素的高敏快速检测。

硫醇和巯基化生物探针已广泛地用于电极表面修饰和化学/生物传感，但采用绿色合成法巯基点击化学制备的巯基化高分子复合材料的生物传感应用目前在文献中鲜见报导。深入研究各种巯基化的新型复合材料（含石墨烯、碳纳米管，纳米金、铂、钯等），利用巯基点击化学中巯基的高反应性赋予复合材料多功能化以提高其富集效率及高效固定生物识别分子，从而赋予生物传感界面放大信号/输出定量信号的功能，创新和优化相关生物传感材料和方法，具有新颖性、学术意义和应用参考价值。在项目中，首先我们在常温条件下简便的制备出了较高品质氧化石墨烯，采用化学预还原-电还原新方法制备出了较高品质多孔石墨烯，采用电生氢气泡模板法制备出了较高品质空心石墨烯；然后采用微波辅助巯基点击制备出了高分散铂纳米颗粒/巯基化聚苯胺-碳纳米管复合物材料，采用电化学法辅助巯基点击制备出了高分散铂纳米颗粒/巯基化聚苯胺-石墨烯复合材料和高分散金纳米颗粒/巯基化聚吡咯-多孔石墨烯复合材料；最后基于上述材料，我们实现了植物激素IAA抗体的高效固定并构建了高敏快速IAA生物传感器，可检测0.2pg/mL-22ng/mL的IAA，其结果与HPLC-MS比较，结果令人满意。项目研究结果对植物激素灵敏快速检测新方法的创建具有重要意义。在本项目培养下，已发表学术论文2篇；即将发表论文6篇；授权国家发明专利2项；申请国家发明专利7项；培养研究生4名；培养优秀本科生10名；项目主持人苏招红已成为湖南省普通高校青年骨干教师培养对象并获得了湖南省教育厅公派出国留学访问一年资格，较好的完成了项目预定的各项目标。