CircPRKD3调控机械力下牙周膜干细胞成骨分化及牙周组织改建的研究

Osteogenic differentiation of periodontal ligament stem cells (PDLSCs) is crucial in orthodontic periodontal tissue remodeling. In previous study, we found that miRNA 21 mediated stretch-induced osteogenic differentiation of PDLSCs by targeting ACVR2B. Based on the function of “miRNA sponges” by circRNAs, we identified circPRKD3 related to miRNA 21 as a potential influential factor during stretch-induced osteogenic differentiation of PDLSCs. Accordingly, our project aims to study the effect of circPRKD3 on stretch-induced osteogenic differentiation of PDLSCs in vitro. In vivo, we will explore the effect of circPRKD3 on orthodontic tooth movement and periodontal tissue remodeling in miRNA21-/- knockout mouse models. Further, we will try to elucidate the interaction of circPRKD3, miRNA 21, parent gene PRKD3 and target protein.This study might demonstrate the function and regulatory mechanism of circPRKD3 during stretch-induced osteogenic differentiation of PDLSCs and periodontal tissue remodeling, which is supposed to provide further strategy for research of physical tooth movement.

牙周膜干细胞成骨分化是正畸牙周组织改建的关键，课题组前期发现miRNA 21通过作用于 ACVR2B调控机械力作用下牙周膜干细胞成骨分化。基于circRNA可作为miRNA“海绵”对其进行负性调控，我们筛选出与miRNA 21相关，在机械力作用下牙周膜干细胞中表达差异最明显的circPRKD3作为研究对象。在此基础上，本课题拟通过体外实验研究circPRKD3对机械力作用下牙周膜干细胞成骨分化的影响；建立miRNA21-/-小鼠正畸牙齿加力模型，观察circPRKD3对牙齿移动速度及牙周组织改建的影响；并探讨circPRKD3与miRNA 21、 parent gene PRKD3及靶蛋白的相互作用。本研究有望阐明circPRKD3对机械力下牙周膜干细胞成骨分化及牙周组织改建的影响及作用机制，为临床促进牙齿健康可控移动提供新的策略。

牙周膜干细胞成骨分化是正畸牙周组织改建的关键，课题组前期发现miRNA 21通过作用于ACVR2B调控机械力作用下牙周膜干细胞成骨分化。基于circRNA可作为miRNA“海绵”对其进行负性调控，我们筛选出与miRNA 21相关，在机械力作用下牙周膜干细胞中表达差异最明显的circPRKD3作为研究对象。在此基础上，本课题拟通过体外实验研究circPRKD3对机械力作用下牙周膜干细胞成骨分化的影响；在机械力负载下过表达或沉默circRNA-PRKD3，观察其对牙周膜干细胞成骨分化的影响，并探讨circPRKD3与其海绵miRNA及靶蛋白的相互作用。本研究有望阐明circPRKD3对机械力下牙周膜干细胞成骨分化及牙周组织改建的影响及作用机制，为临床促进牙齿健康可控移动提供新的策略。