中国龟象亚科分类修订及族级系统发育关系重建

As a subfamily of Curculionidae in Coleoptera, Ceutorhynchinae is distributed almost worldwide, with 1337 species in 173 genera and 11 tribes recorded. Because of their high host specificity, great diversity, wide distribution, tiny body size and distinct morphological similarity, identification of the taxon is never been easy. Phylogenetic work on the group is still very limited, and hardly any characters are known that clearly suggest the monophyly of some subgroups above the generic level. Many tribes are even not clearly defined. Based on the previous records and new materials examined, 219 species in 56 genera and 10 tribes are discovered from China. This proposal plans to work on systematics of Ceutorhynchinae from China, based on taxonomic revision in generic level and combined morphological and molecular phylogeny in tribal level. At least 32 major genera would be reviewed and four mitochondrial genes of 50 representative species would be sequenced and analyzed as well as the morphological data to reconstruct phylogenetic relationships within the subfamily. The results will promote the systematic research of the subfamily and prepare the further studies into the coevolutionary relationships between the ceutorhynchine weevils and their specified host plants.

龟象亚科Ceutorhynchinae是鞘翅目象虫科中呈世界性分布的一个亚科，全世界已记录11族173属1337种。由于寄主专一性高、个体较小、种间形态差异小，种类鉴定困难，影响了对种间、属间、族间关系的深入了解。亚科现行分类系统无法体现族级甚至属级分类阶元的系统发育地位，多数族的范畴及单系性存在较大争议。申请人综合文献记录和检视标本，发现中国龟象亚科昆虫已知和未定名的种类共10族56属219种，亟待深入系统分类修订。本项目拟全面系统研究中国龟象亚科分类及系统发育，重点系统分类修订32属，并从形态学角度进行属级识别的形态指标筛选；同时采用4个线粒体基因序列为分子标记，选取50个代表性物种，分析其系统发育关系；综合形态和分子系统学证据，分析并解决龟象亚科各族的单系性问题，重建各族的范畴，明确重建后各族的系统发育关系，为深入研究龟象昆虫与寄主植物的相互关系及协同进化等科学问题奠定基础。

龟象亚科Ceutorhynchinae是鞘翅目象虫科中呈世界性分布的一个亚科，由于寄主专一性高、个体较小、种间形态差异小，种类鉴定困难，影响了对种间、属间、族间关系的深入了解。项目结合形态特征与线粒体基因序列，对中国龟象亚科昆虫展开系统发育与分类研究。通过广泛的国际合作，对白点龟象属Cardipennis等属采用形态学和生物学信息完成属级厘定，提供属级及物种的鉴别特征、物种描述和高清形态特征图。结合形态和DNA条形码数据的对蓼龟象属Phytobius等属展开物种的分子鉴定与形态特征研究，提取DNA条形码，发现不同地理种群的形态变异等，初步建立龟象亚科昆虫物种分子鉴定的参考遗传距离，并为此类昆虫的快速分子鉴定提供了依据。综合形态、分子和生物学信息等，展开族级系统发育研究，解决蓼龟象族和刺龟象族等族争议问题，重建该两族的范畴，并进一步探讨各族中存在的问题，完善和修订龟象亚科的分类系统，为深入研究龟象昆虫与寄主植物的相互关系及协同进化等科学问题奠定基础，并为龟象昆虫用于入侵杂草的生物防治和相关龟象害虫的综合治理提供了支持。.项目研究中，依据分子数据和形态特征，通过对各属的详细厘定和对已记录种类的整理，共记录中国龟象亚科昆虫10族51属146种，包括1新属、16新种、18新记录种。已经发表研究论文6篇，并尚有大量研究结果即将发表；培养硕士研究生2人。.