油菜矮杆新基因BnDF1在GA信号转导途径中的功能分析

Plant height is closely related to lodging and mechanized harvesting in rapeseed. Exploration and research of dwarf recourses is of great significance for breeding of dwarf rapeseed. In previous study, a dwarf mutant, m61, was identified through EMS mutagenesis of Brassica napus L. cultivars Zhongshuang 11. The dwarf trait was controlled by a single dominant gene, BnDF1, which was mapped into a 1.31 Mb interval on chromosome C05. The m61 was gibberellin insensitive and the genes in the GA signal transduction pathway were significantly down-regulated in the mutant. A follow-up study of the project will clone BnDF1 by whole genome sequencing and Mutmap analysis, identify the biological function of BnDF1, survey GA signaling regulatory networks which the BnDF1 gene involves, and reveal the molecular mechanism of dwarf plants through the regulation of GA signal pathway.

油菜株高与倒伏和机械化收获密切相关。油菜矮杆资源的挖掘及研究对油菜矮化育种具有重要意义。我们通过EMS诱变甘蓝型油菜品种中双11号，获得一个稳定遗传的矮杆突变体m61，矮化性状受一对显性基因BnDF1控制，定位于甘蓝型油菜C05染色体上1.31 Mb区间内，与目前已定位的油菜矮杆基因不在同一位点。该突变体对赤霉素不敏感，赤霉素信号转导途径基因在突变体中显著下调表达。本项目后续研究将采取全基因组重测序并结合Mutmap分析方法，定位克隆矮杆基因BnDF1，并深入解析BnDF1的生物学功能，调查BnDF1基因参与的GA信号转导调控网络，并最终揭示BnDF1通过调控GA信号转导而导致植株矮化的分子机理。

油菜株高与倒伏和机械化收获密切相关。油菜矮杆资源的挖掘及研究对油菜矮化育种具有重要意义。本研究前期通过EMS诱变甘蓝型油菜品种中双11号，获得一个稳定遗传的矮杆突变体m61，其矮化性状受一对显性基因BnDF1控制，被定位于甘蓝型油菜C05染色体上1.31 Mb区间内。基于以上研究背景，本项目开展了四个方面的研究内容：1）BnDF1基因的精细定位与克隆；2）BnDF1基因的表达模式和亚细胞定位分析；3）油菜BnDF1基因的生物学功能鉴定；4）BnDF1基因参与的调控网络调查。通过本项目的实施，现已将BnDF1基因精细定位并克隆，验证了BnDF1 基因可以调控油菜株高的功能，解析了BnDF1通过调控GA信号转导而导致植株矮化的分子机理。本研究可望为油菜矮杆育种提供基因资源，为油菜矮化分子机制研究提供有价值的信息。