榛子高密度遗传连锁图谱的构建及重要农艺性状QTL定位

The industry of horticultural cultivation of hazelnut is weak in china,selective breeding and popularization slowly,high density genetic linkage map of hazelnut is important to improve the efficiency of breeding and speed up the industry process of hazelnut；At the same time,confirm hazelnut QTL mapping of agronomic traits,which will be further provides the theory basis for the molecular marker-assisted selection.We used Corylus heterophylla Fisch × C．avellana to backcross with Corylus heterophylla Fisch, established a segregating population of hybrid offspring of hazelnu which have wider variation,breeding new varieties with higher yield and quality to carry out hybridization breeding effectivelt for effectively further development of cross-breeding, in order to intensive study of the genetic basis of the Corylus heterophylla Fisch and European hazel,we need to find out the reason of differ of yield and quality among interspecific hybrid and intervarietal，and to carry out hazelnut collection of important agronomic genes is the basis to carry out the above work. In this study, we will construction of hazelnut SSR and SNP molecular genetic map, and QTLs controlling cold resistance,Nut mass,Kernel mass,percentage,nut thickness and Yield per tree of imporant agronomic traits were detected,and fill the gaps of construction of hazelnut high density genetic linkage map at international.

我国榛子园艺化栽培产业基础薄弱，优良品种选育和推广缓慢，榛子高密度遗传连锁图谱的构建对提高新品种选育效率，加快榛子产业化进程有重要作用；同时，确定榛子重要农艺性状的QTL定位，将为进一步开展其分子标记辅助育种等提供理论依据。我们以种间杂种平欧杂交榛作亲本与延边长白山脉野生平榛优系进行回交，建立了一个杂种后代产生更大范围变异的复杂分离群体，选育出了具有更高产量和品质的新品种，为了有效地进一步开展杂交育种工作，深入研究平榛与欧洲榛种间杂交种的遗传基础，需要找出导致种间杂种产生种子和品种间产量存在差异的本质原因，榛子重要农艺基因的收集是开展以上工作的基础。本研究拟构建SSR和SNP分子标记遗传连锁图谱,并分析群体抗寒性、单果重、果仁重、出仁率、果壳厚度以及单株产量等农艺性状进行QTL定位，此研究将填补国内外榛子高密度遗传连锁图谱构建的空白。

我国榛子园艺化栽培产业基础薄弱，优良品种选育和推广缓慢，榛子高密度遗传连锁图谱的构建对提高新品种选育效率，加快榛子产业化进程有重要作用；同时，确定榛子重要农艺性状的QTL定位，将为进一步开展其分子标记辅助育种等提供理论依据。我们以种间杂种平欧杂交榛作亲本与延边长白山脉野生平榛优系进行回交，建立了一个杂种后代产生更大范围变异的复杂分离群体，选育出了具有更高产量和品质的新品种，为了有效地进一步开展杂交育种工作，深入研究平榛与欧洲榛种间杂交种的遗传基础，需要找出导致种间杂种产生种子和品种间产量存在差异的本质原因，榛子重要农艺基因的收集是开展以上工作的基础。本研究构建了SNP分子标记遗传连锁图谱,开发465,427 个SLAF 标签，上图标记亲本平均深度168.79X，子代42.27X，图谱密度高。并分析群体抗寒性、单果重、果仁重、出仁率、果壳厚度以及不饱和脂肪酸等农艺性状进行QTL定位，此研究填补了国内外榛子高密度遗传连锁图谱构建的空白。