鸡抗新城疫病毒感染过程中的RLRs抗病毒信号通路分子机制研究

Viral disease is greatly harmful to the development of poultry industry; deep studies on antiviral signal pathway in chicken will be useful for breeding for disease resistance. RIG-I-like receptors(RLRs) is the first intracellular viral recognizing receptor that trigger the production of interferon through MAVS activating IRF-3 (interferon-regulated factor 3) and NF-κB(Nuclear Factor-κB), which finally inhibit the viral replication, It has been known that RLRs-mediated signaling pathway plays a crucial role in mammals response to virus infection. However, its role in chicken remains unknown. In present study, firstly, we enable chicken fibroblast cell and macrophages to be infected with Newcastle disease virus(NDV) for investigating the molecular mechanism under RLRs-mediated antiviral signal pathway in chicken response against NDV infection. Secondly, we study the relationship between V protein of NDV and RLRs-mediated signaling pathway, and clarify the molecular mechanism of V protein-inhibited host interferon production. Finally, SPF layer are chosen for infection with NDV, in order to prove the role of the RLRs-mediated antiviral pathway in vivo of chicken response to NDV. Consequently, the role of RLRs-mediated antiviral signaling pathway in chicken antiviral immune process can be explained. Furthermore, as molecular markers, the related genes of RLRs-mediated signaling pathways will provide a theoretical basis for the selection of disease-resistant traits in poultry breeding.

病毒性的疾病严重危害家禽养殖业的发展，深入的探究家禽的抗病毒信号机制可为以后的家禽抗病育种打下基础。细胞内的RLRs病毒识别受体能够通过MAVS蛋白激活细胞中的多种衔接因子诱导干扰素基因的表达，从而抑制病毒的复制，其在哺乳动物的细胞和体内抗病毒免疫过程中发挥着重要的作用，但其在家禽体内的作用仍不明确。本试验首先通过鸡胚成纤维细胞和巨噬细胞攻毒试验，在细胞水平探索RLRs介导的抗病毒信号通路在鸡抗新城疫病毒免疫过程中的分子机制，然后进一步研究新城疫病毒的V蛋白与鸡的RLRs抗病毒信号通路之间的相互关系，最后通过机体攻毒试验证实这条抗病毒信号通路在鸡体内抗新城疫病毒感染过程中的作用。研究结果将使我们更好认识RLRs介导的抗病毒信号通路在家禽的抗病毒免疫过程中的作用，并为将RLRs通路中的相关基因作为家鸡抗病性状选育的分子标记提供理论依。

禽养殖业的发展一直受到病毒性疾病的危害，本试验深入的探究家禽的抗病毒信号机制可为以后的家禽抗病育种打下基础。细胞内的RLRs病毒识别受体能够通过MAVS蛋白激活细胞中的多种衔接因子诱导干扰素基因的表达，从而抑制病毒的复制，其在哺乳动物的细胞和体内抗病毒免疫过程中发挥着重要的作用，本试验首先通过鸡胚成纤维细胞和巨噬细胞攻毒试验，在细胞水平探索RLRs介导的抗病毒信号通路在鸡抗新城疫病毒免疫过程中的分子机制，然后进一步研究新城疫病毒的V蛋白与鸡的RLRs抗病毒信号通路之间的相互关系，最后通过机体攻毒试验证实这条抗病毒信号通路在鸡体内抗新城疫病毒感染过程中的作用。研究结果将使我们更好认识RLRs介导的抗病毒信号通路在家禽的抗病毒免疫过程中的作用，并为将RLRs通路中的相关基因作为家鸡抗病性状选育的分子标记提供理论依。