OsWRKY71在水稻抗褐飞虱基因Bph15抗虫信号途径中的功能研究

The brown planthopper (Nilaparvata lugens Stål, BPH) is one of the most destructive pests in rice (Oryza sativa L.) production. To date, three BPH-resistance genes (Bph14, Bph3 and Bph26) have been successfully cloned. But, the resistance gene-regulated molecular mechanism still remains unknown. WRKY domain containing transcription factors play important roles in disease resistance of rice. However, roles of WRKY transcription factors in BPH-responses of rice have not yet been reported. Bph15 is an important BPH-resistance gene. In the previous work, we wanted to elucidate the defense mechanism of Bph15. OsWRKY71 was upregulated after BPH infestation in the resistant genotype containing Bph15 gene. No significant difference was observed in susceptible plants. In this project, we will dissect the function of OsWRKY71 in the insect-resistant signaling pathway mediated by Bph15 in rice using transgenic technique. To elucidate the molecular mechanism by which OsWRKY71 regulates the BPH resistance, we will screen the proteins interacting with OsWRKY71 and genes acting downstream of OsWRKY71 in rice. This work not only provides insights into the molecular mechanisms of Bph15 but also the interaction between the plant and piercing-sucking insects.

褐飞虱是危害水稻生产最严重的害虫之一。目前Bph14、Bph3和Bph26三个抗褐飞虱基因已被成功克隆，但抗褐飞虱基因调控水稻抗褐飞虱的分子机制和调控途径仍不清楚。WRKY转录因子在水稻抗病反应中有重要作用，然而尚未有其参与水稻抗褐飞虱反应的相关报道。Bph15是重要的水稻抗虫基因位点。在研究Bph15水稻抗虫机理过程中，我们发现OsWRKY71基因在抗虫材料中受褐飞虱取食显著上调表达，而在感虫材料中没有显著变化，这暗示OsWRKY71可能参与了Bph15抗虫途径。本项目拟通过转基因手段验证OsWRKY71在抗褐飞虱基因Bph15抗虫信号途径中的作用，并鉴定OsWRKY71的互作蛋白质以及下游调控基因，解析OsWRKY71参与的Bph15抗褐飞虱信号通路。研究结果可为阐明抗褐飞虱基因Bph15的抗虫机理提供依据，为研究植物与刺吸式口器昆虫的相互作用及调控机理提供参考。

褐飞虱是危害水稻生产最严重的害虫之一。Bph15是重要的水稻抗虫基因位点，但其调控水稻抗褐飞虱的分子机制和调控途径仍不清楚。WRKY转录因子在水稻抗病反应中有重要作用，然而尚未有其参与水稻抗褐飞虱反应的相关报道。本研究证明OsWRKY71在抗褐飞虱基因Bph15抗虫信号途径中起关键作用，探索了OsWRKY71参与的Bph15抗褐飞虱信号通路，从microRNA角度分析了Bph15的抗虫机理，开发了功能性SNP分子标记。研究结果可为阐明抗褐飞虱基因Bph15的抗虫机理提供依据，为研究植物与刺吸式口器昆虫的相互作用及调控机理提供参考。