基于"料液流变特性与膜萃取传质关系"的超滤-膜萃取耦合技术分离黄酮类物质研究

The technology used to separate flavonoids from the extraction solution of Chinese traditional medicine includes the liquid-liquid extraction, macroporous resin adsorption and polyamide absorption at present. But the existing technology have some disadvantages in different extent, such as the complex process, high solvent consumption, high cost, low purity of the product and residual organic solvent in the product. The technology of liquid-liquid extraction will not be used to separate the natural substances because of the serious emulsion caused by the surface activesubstance contained in the extraction solution of Chinese traditional medicine. The coupling technology of ultrafiltration coupling solid membrane extraction will be used as the key technology, which was created by our project team, and glycyrrhiza will be used as the study material that contains the surface activesubstance and many kinds of flavonoids. We will study the coupling relationship of the key technology by study the relationship of the liquid materials rheological property and the mass transfer of solid membrane extraction and will create the correlation for the overall mass transfer coefficient of simultaneous membrane extraction and back extraction. We will improve the applicability of the key technology and will provide a new technology for the separation process of flavonoids to overcome the disadvantages of the existing technology. The correlation can provide the value reference for the separation process of other natural substances when using the coupling technology. The study result can provide a new applicable technology for the deep processing of glycyrrhiza also.

中药提取液中黄酮类物质的分离目前主要有液液萃取、大孔树脂吸附、聚酰胺吸附等技术。但已有技术均不同程度的存在工艺复杂、有机溶剂用量大、成本高、产品纯度较低及有机溶剂残留等问题。特别当提取液中含有表面活性物质时最常用的液液萃取技术将因严重乳化无法使用。 本项目以黄酮种类较全且含有表面活性物质的甘草为研究对象，以项目组创建的超滤-固体膜萃取耦合技术为关键技术，以超滤料液流变特性与膜萃取传质关系的研究为切入点，建立包含料液流变特性参数的同级膜萃取反萃取总传质系数关联式，通过深入研究耦合技术两个组成单元间的耦合关系来提高其分离黄酮类物质的适宜性。适用性提高后的耦合技术在较大程度上可克服现有技术存在的缺陷，为黄酮类物质的分离提供新的适用技术。研究所得关联式对使用该耦合技术分离其他天然物质也有一定参考价值。 项目研究结果还将为甘肃地道药材甘草的精深加工提供新的适用技术。

本项目主要完成了甘草提取工艺优化研究、甘草超滤单元相关研究（包括超滤工艺的优化、浓缩对超滤液的流体特性影响研究、料液由牛顿流体向非牛顿流体转变时浓缩终点的确定。浓缩程度与料液雷诺数、跨膜压力间关系的研究）。完成了膜萃取单元相关研究（包括萃取剂的临界pH值的确定、萃取膜的选择研究；同级萃取反萃取膜组件的制备及萃取过程设计、膜组件装填密度的确定、总传质系数关联式确定），完成了超滤-膜萃取耦合技术分离黄酮类物质条件初步确定、确定了甘草总黄酮的膜萃取条件。 . 由于目前黄酮类物质分离所用液液萃取、大孔树脂吸附、聚酰胺吸附等技术存在的工艺复杂、有机溶剂用量大、成本高、产品纯度较低及有机溶剂残留等问题。本项目以超滤-固体膜萃取耦合技术为关键技术，以甘草为模型药材，通过系统研究超滤和固体膜萃取两项技术间的耦合关系以及黄酮类物质的传质情况与超滤料液流变特性间的关系，为黄酮类物质的分离提供新的适用技术。同时为甘肃地道药材甘草的精深加工提供新技术支撑。