单分散J聚合物液晶液滴生物传感器的研究

Liquid crystal (LC) droplets have great potential for sensor applications because of their large surface areas, tunable surface properties and well-defined director configurations. They are stimuli-responsive soft materials, with the adsorption of chemical and biological species at the interface may change the surface anchoring energy and trigger the configuration transition of the LC droplets. . The aim of the project is to fabricate monodisperse LC droplets with innovative surface modification by cyanine dyes and applications for sensing dopamine. A key point of the project is the fabrication of LC droplets with good monodispersity, which is essential for the sensor applications by controlling the dispersed phases. The resulting monodispersed LC droplets will be surface modified by cyanine dye molecules. Cyanine dyes can be assembled into J-aggregates, in which electronic excitations are coherently delocalized over many dye molecules rather than confined at individual molecules. The resulting systems will be characterized by a combination of Polarized and Fluorescence Microscope, UV/Vis and Fluorescence Spectroscopy, Cyclic Voltammetry, TEM, SEM, AFM, and so on. Spectroscopy and microscopy are the key techniques used in this project, thus, allowing deep insight into the structure and properties of the materials. The structural and spectroscopic properties will be resolved together, allowing for determining exactly how the ordering of the dye molecules affect the electrochemical and photoelectrical properties of the materials, which are essential for the detection of dopamine.. This outlook for an innovative liquid crystal biosensor for detecting dopamine makes the proposal very important for the future of liquid crystal research in soft matter science and engineering.

本课题将利用单分散液晶液滴乳液，通过菁染料使其表面功能化，制备具有独特光电特性的J聚合物液晶液滴材料，开展对多巴胺的探测研究。研究微流控芯片设计，制备具有良好单分散性的液晶液滴；研究使用菁染料对液晶液滴表面功能化的技术，并研究菁染料在液晶表面自组装结构对所形成J聚合物光电特性的影响；研究单分散J聚合物液晶液滴对多巴胺探测的敏感度和选择性。本课题的工作将为今后我们探索设计新型液晶生物传感器提供重要实践和理论基础；并通过结合显微镜法，光谱分析法和电化学分析方法得到快速和精确的探测结果，拓宽液晶传感器在软材料科学与工程领域的发展前景。

发展高敏感度且高选择性的光学传感器在软材料科学与工程领域具有重要的发展前景。本课题通过材料的表面与界面相互作用，设计出了制备简易、反应速度快、选择性好且检测极限高的光学传感器。通过使用聚合物框架，通过固液相分离的方法制备出聚合物分散液晶液滴气体传感器，并对液晶液滴的大小，分布，形状以及矢向量进行调节。通过甲苯气体与构成聚合物框架的聚合物之间强的相互作用，诱导液晶液滴发生相变，实现了对甲苯气体的选择性检测。本项目还通过J聚合物的特殊光学特性，实现了在模拟人体尿液中对石胆酸的快速检测，并对次级胆汁酸中同时并存的胆酸和熊去氧胆酸没有响应，展示了良好的选择性。此外，本项目合成出具有良好生物相容性的荧光传感器，开展了在活细胞中对半胱氨酸和肼的检测，制备出了两种具有良好荧光响应的生物传感器。本课题集中研究了基于材料表面与界面相互作用的光学生物传感器，并对光学传感器的设计与机理进行了深入的研究与讨论，为进一步开拓该技术的发展提供了基础。