外周血EPC和MSC的共培养复合3D打印聚乳酸支架/胶原水凝胶构建血管化组织工程半月板

Meniscal repair and reconstruction has always been the main problem in the field of orthopedic sports medicine. Currently the latest research shows that tissue engineering meniscus provides a solution for this problem. Our preliminary study found that the selection of seed cells and scaffold and their outcome in vivo played an important role in their clinical application. Based on successfully mobilizing and minimally invasive obtaining endothelial progenitor cells (EPC) and mesenchymal stem cells (MSC) from peripheral blood, we build the co-culture system of peripheral blood EPC and MSC under 2D and 3D micromass pellet by simulating the interaction of meniscal cells and endothelial cells in the natural 1/3 lateral meniscus, and screen the optimal EPC/MSC ratio from the cell level. The vascularized engineering meniscus is constructed with the co-culture of peripheral blood EPC and MSC composited 3D printing polylactic acid scaffold / collagen hydrogel, which will provide the basis for long-term survival and outcome of tissue engineering meniscus in vivo.

半月板损伤修复与重建一直是骨科及运动医学面临的主要难题，最新研究表明，组织工程化半月板为这一难题解决提供了楔机。我们前期研究发现，种子细胞的选择及其在体内的转归、支架材料的筛选等是工程化半月扳临床应用面临的关键问题。本研究拟在从成功动员并微创分离外周血内皮祖细胞（EPC)和间充质干细胞（MSC）的基础上，通过模拟天然半月板外侧1/3组织半月板细胞和内皮细胞相互作用微环境，在外层浸入EPC/MSC用于模拟半月板外侧1/3血管化工程半月板，内层浸入MSC用于模拟半月板内侧2/3无血管区；构建外周血来源EPC与MSC二维和三维微团共培养体系，从细胞水平筛选最佳的EPC/MSC配比；通过外周血来源EPC/MSC共培养及复合3D打印聚乳酸多孔支架/胶原水凝胶，构建血管化工程半月板，促进组织工程半月板在体内的长期存活和优化转归，为临床半月板损伤的治疗提供新的策略。

半月板损伤修复与重建一直是骨科及运动医学面临的主要难题，最新研究表明组织工程半月板为这一难题解决提供了楔机。本课题通过联合药物动员方法促进外周血干细胞的动员释放，通过形态、表面标记物、三系分化潜能鉴定为MSCs；研究外周血间充质干细胞（peripheral blood-derived marrow stem cells, PBMSCs）与生物相容性及生物力学良好的脱钙皮质骨基质（demineralized cortical bone matrix，DCBM）支架材料相互作用的生物学特点；探讨DCBM支架材料和TGF-β3在体外对兔PBMSCs的成纤维软骨细胞的诱导效果；进一步探讨DCBM支架材料和PBMSCs构建的组织工程半月板对兔内侧半月板修复再生的效果以及对关节软骨的保护情况。本研究成功促进自体内在干细胞向外周血释放，并联合支架材料和生长因子TGF-β3体内外修复兔内侧半月板取得较好的效果，但组织工程半月板的种子细胞及3D打印支架的梯度化差异构建等方面尚需要进一步深入研究。