多色荧光氧化石墨烯试纸的可视化分析方法

Paper sensors possess many advantages, such as visual analysis, low-cost, fast and portable feasibility. However, tranditional fluorescent paper sensors are not applicable due to photo-bleaching, bad stability and difficult to fabricate.In this project, we will design and prepare multicolour photoluminescent graphene oxide ink to print test paper for simultaneous and visual detections of ultrace and highly specific tumor marker proteins in the serum of cancer patients by the employment of water-soluble and optical properties-stable fluorescent graphene oxide. First, we will explore methods to tune the sizes of graphene oxide nanosheets and prepare red, yellow and green-emitted graphene oxide through surface functionalization. Second, we will develop a fluorescence off-to-on analytical principle based on fluorescent resonance energy transfer, and research the selectivity and sensitivity of the fluorescent switch. At last, we will prepare the multi-colored fluorescent graphene oxide ink, and print it onto paper-like substrates to fabricate test paper for the simultaneous, fast and visul detections of tumor marker proteins. According to this, we will establish the visual analytical methods of fluorescent graphene oxide paper sensors. The expected results of this project will provide scientific basis and technical guidance for the fast and visual analysis of tumor marker proteins by paper sensors.

试纸传感器具有可视化、低成本和快速便携等优点，传统的荧光纸质传感器由于光漂白，稳定性差及试纸制备困难等问题而无法得到实际应用。本项目拟利用荧光氧化石墨烯发光稳定和水溶性好等优点，设计和制备多色荧光氧化石墨烯的功能化墨水，印刷多色氧化石墨烯试纸，实现对多组分肿瘤标志蛋白的同步可视化检测，具体研究内容如下：（1）探索氧化石墨烯尺寸的可控调节方法，并通过表面功能化，制备红、黄、绿三种典型颜色的水溶性荧光氧化石墨烯；（2）发展基于共振能量转移的荧光开关检测原理，研究荧光开关对肿瘤标志蛋白的选择性和敏感性；（3）制备多色荧光氧化石墨烯功能墨水，通过喷墨打印构建出多色荧光氧化石墨烯试纸，实现对肿瘤标志蛋白的同步、快速、可视化分析，建立基于荧光纸质传感器的可视化分析方法。本项目的预期成果将为肿瘤标志蛋白的快速可视化分析提供科学依据与技术途径。

本项目面向痕量目标物快速实时检测的需求，利用氧化石墨烯、上转换纳米颗粒等纳米材料独特的光学性质，制备出兼有选择性识别能力和敏感信号输出的新型荧光探针，并将其组装成纸质传感器，很好地克服了传统荧光试纸面临的易光漂白，灵敏度差等难题，实现了痕量物质的快速可视化分析。主要研究成果如下：（1）发展了一种新的化学合成方法，可控地将氧化石墨烯纳米片的荧光从红棕色调控到黄色、绿色和青色，揭示了氧化石墨烯发光颜色与尺寸之间的关系，并利用其构建一些荧光开关，实现痕量目标物的快速检测；（2）发展了几种新的试纸传感器，借助智能手机和3D打印技术实现试纸上发光信号的准确获取和解析，用于目标物快速、定量、可视化的检测；（3）探索了几种新的上转换发光纳米探针，并将其用于药物活性的可视化评估，细胞内活性氧物种的痕量检测等等。本项目共发表研究论文5篇，其中影响因子大于5.0的4篇，获得国家授权发明专利2项。