基于直接电离质谱的人体实际烟气有机物暴露评价的新方法研究

The current risk assessments of mainstream cigarette smoke have not considered the actual amounts of the organic compounds exposed to human organs, and thus may not accurately reflect the situation of real smoking process. To solve this problem, this project aims to develop a new method based on direct ionization mass spectrometry for human actual exposure assessment of organic compounds during cigarette smoking. In this project, an online analysis mass spectrometry platform will be developed using a single photon ionization mass spectrometry (SPI-MS) coupled with an extractive electrospray ionization mass spectrometry (EESI-MS). A sampling system will be designed to enable automatic sampling of cigarette smoke to both mass spectrometry instruments. The experimental conditions of SPI-MS and EESI-MS will be optimized to establish an online analysis method of organic compounds in cigarette smoke. Smoking volunteers will be recruited to generate inhaled smoke and exhaled smoke, and then the smoke will be analyzed by the established platform. Finally, according to the online analysis results of inhaled smoke and exhaled smoke, a human exposure model of organic compounds in cigarette smoke will be established. This project will provide a new idea for exposure assessment researches on organic compounds in cigarette smoke, and thus is of great value for the researches on smoking and health issues.

以卷烟主流烟气为对象的吸烟危害评价，没有考虑人体内烟气有机物的实际接受量，反映的人体吸烟过程与真实情况可能存在差异。本申请针对卷烟抽吸过程中人体实际烟气有机物暴露研究的需要，发展基于直接电离质谱的人体实际烟气有机物暴露评价的新方法。本项目拟将单光子电离质谱（SPI-MS）和电喷雾萃取电离质谱（EESI-MS）相结合，构建直接电离质谱的在线分析平台；设计烟气进样系统，实现烟气样品的直接质谱进样；优化SPI-MS和EESI-MS的实验条件，建立烟气有机物的在线分析方法；选取吸烟志愿者，对卷烟抽吸过程中人体吸入和呼出的烟气进行在线分析。最终，根据吸入烟气和呼出烟气的在线分析结果，建立卷烟抽吸过程中人体烟气有机物的暴露模型。本项目发展的直接电离质谱在线烟气分析方法，为烟气有机物暴露的研究提供了新思路，在吸烟与健康研究方面具有很强的应用价值。

本项目建立了基于单光子电离飞行时间质谱的烟气气相成分在线分析方法，设计了针对人体吸入和呼出烟气的质谱采样系统，对吸烟者吸入和呼出的烟气取样，并直接引入至光电离源中进行电离；通过吸烟者抽吸卷烟实验，获得了卷烟抽吸过程中三十多种烟气气相成分的人体残留率。发展了电喷雾萃取电离源，与离子阱质谱相结合，设计了人体呼出烟气的进样系统，建立人体呼出烟气的在线分析方法，通过吸烟者抽吸卷烟实验，获得了烟气粒相中烟碱和茄尼醇的人体残留率。建立了基于电迁移粒径谱仪的气溶胶测试方法对卷烟烟气气溶胶进行测试，气溶胶数据结合多通道沉降模型模拟了烟气气溶胶在人体的沉降。本项目将为吸烟与健康研究提供重要的数据支撑。