磁性石墨烯的制备及其在烟草特有N-亚硝胺分析中的应用

To assess exposure of non-smokers to tobacco-specific N-nitrosamines (TSNAs) in the environment, it is necessary to monitoring of TSNAs release in mainstream smoke. However, some disadvantages in previous reports for the analysis of TSNAs in mainstream smoke were existed, such as tedious sample preparation procedure, serious matrix interferences and inaccurate quantitation. To solve the above problems, this project intends to introduce magnetic solid-phase extraction (MSPE) technology into tobacco and tobacco products analysis, and then propose a new rapid, rapid, green and facile synthesis approach of high-quality graphene in large-scale. Functionalized magnetic materials based on graphene will be synthetized and characterized. The magnetic materials will be used as MSPE sorbent for the fast enrichment of trace TSNAs in mainstream smoke. By employing highly sensitive instruments, rapid, effective, sensitive, accurate determination method of trace TSNAs in mainstream smoke will be achieved. The implementation of this project will be not only provides a new analytical method for disclosure and regulation of harmful components release in tobacco products, but provides technical support for assess exposure of non-smokers to TSNAs in the environment.

为了评估不抽烟者对环境中烟草特有N-亚硝胺的暴露量，有必要对主流烟气中烟草特有N-亚硝胺的释放量进行测定。目前文献报道的主流烟气中烟草特有N-亚硝胺释放量的测定方法普遍存在样品前处理操作繁冗费事、基质干扰严重和定量不准确等问题。为了解决上述问题，本项目拟将磁固相萃取技术引入烟草及烟草制品分析中，从高质量石墨烯的快速、绿色、温和的制备新方法出发，进而合成和表征磁性石墨烯材料并对其吸附性能进行测试，最后将其用作磁固相萃取吸附剂实现主流烟气中痕量烟草特有N-亚硝胺的快速分离、富集；结合色谱-质谱等分离检测手段，建立主流烟气中烟草特有N-亚硝胺释放量的快速、高效、灵敏、准确的分析检测方法。本项目的实施不仅为披露和管制烟草制品中TSNAS释放量提供新的分析手段，还能为评估不抽烟者对环境中烟草特有N-亚硝胺的暴露量提供技术支持。

烟草特有N-亚硝胺是卷烟主流烟气中重要的释放物，为了准确、快速测定卷烟主流烟气中烟草特有N-亚硝胺的释放量，本项目进行了以下工作：（1）以食品抗氧化剂为还原剂制备并表征石墨烯，该方法具有安全绿色、可以避免使用在传统制备方法中不可缺少的有毒化学试剂、制备成本低等优点；（2）针对不同官能团修饰石墨烯，通过比较其对烟草特有N-亚硝胺的吸附效果对其进行筛选，最后得到适合的石墨烯类型；（3）通过简单的物理混合制备磁性石墨烯吸附剂，并研究其对烟草特有N-亚硝胺的吸附机理，结合液相色谱-串联质谱，建立了卷烟主流烟气中烟草特有N-亚硝胺释放量的快速、高效、灵敏、准确的分析检测方法。本项目制备的磁性石墨烯材料有望实现产业化，不仅为分析烟草制品中烟草特有N-亚硝胺释放量提供新的分析手段，更是石墨烯在烟草特有N-亚硝胺分析中的首次尝试。