六溴环十二烷微生物降解的分子机理研究

The brominated flame retardants (BFRS) are widely used in textile, plastics, electronics and building materials, and will take up almost 80％ of all fire retardants. Hexabromocyclododecanes (HBCDs) (the third of world’s used flame retardants) is listed on persistent organic pollutions under the Stockholm Convention. It has attracted a worldwide attention on account of its persistence, biological cumulative and toxicity. Therefore, high efficient degradation of HBCDs in environment has great significance on human health and environmental protection. In recent years, many microbes or strains can degrade HBCDs in aerobic or anaerobic condition. Reported work has limited on the degrading ability, while, there is almost no report about the degradation pathways, molecular mechanism and biochemical mechanism. Strain Pseudomonas aeruginosa HS9, which can degrade HBCDs (α-, β- and γ-HBCD) will be taken as the research object. This proposed project will be dedicated to systematic investigation on the complete pathway of HBCDs. To identify the key function genes and catalytic mechanism in this pathway, with the help of multiple omics analysis, such as genome sequencing, transcriptome analysis and differential proteomics analysis. The deeply research on molecular mechanism of HBCDs degradation will provide important scientific and practical significance for bioremediation of HBCDs.

溴代阻燃剂被广泛的应用于纺织品、塑料、电子和建筑材料中，占阻燃剂使用量的80％。六溴环十二烷（HBCD）（全球使用量第三的阻燃剂）被欧洲化学品管理局列入“高关注度化学品清单”。由于具有持久性、生物积累性和毒性，导致的环境问题日益突出，已经在全球范围内引起广泛的关注。高效降解环境中的HBCDs对于维护人类健康和环境保护具有重要意义。近年来，人们发现了在好氧或厌氧下降解HBCDs的菌群或菌株，仅仅局限于降解能力上，其降解途径、分子机理、生化机制的报道几乎没有。本项目拟以筛选获得的一株具有HBCDs（α-、β-和γ-HBCD）降解能力的铜绿假单胞菌HS9为研究对象，鉴定菌株HS9的HBCDs降解途径；通过基因组、转录组和差异蛋白组学等多组学手段，寻找HBCDs降解关键基因，研究关键酶催化机理。本项目将深度研究HBCDs降解的分子机理，对生物修复具有重要科学与实践意义。

溴代阻燃剂被广泛的应用于纺织品、塑料、电子和建筑材料中，占阻燃剂使用量的80%。 六溴环十二烷(HBCD)(全球使用量第三的阻燃剂)被欧洲化学品管理局列入“高关注度化学 品清单”。由于具有持久性、生物积累性和毒性，导致的环境问题日益突出，已经在全球范围 内引起广泛的关注。高效降解环境中的HBCDs对于维护人类健康和环境保护具有重要意义。近年来，人们发现了在好氧或厌氧下降解HBCDs的菌群或菌株，仅仅局限于降解能力上，其降解 途径、分子机理、生化机制的报道几乎没有。本项目以筛选获得的一株具有HBCDs(α-、β -和γ-HBCD)降解能力的铜绿假单胞菌HS9为研究对象，鉴定了菌株HS9的HBCDs降解途径;通过基因组、转录组和差异蛋白组学等多组学手段，寻找到HBCDs降解关键基因，并研究了关键酶催化机理。本项目将深度研究HBCDs降解的分子机理，对生物修复具有重要科学与实践意义。