广藿香根系分泌物与根际微生物间的互作效应及分子机制研究

Pogostemon cablin has been one of the well-known Chinese medicinal materials. The essential oil from this herb is effective to "transform turbid dampness, stop vomiting and stimulate appetite, release exterior dampness and summer-heat attack". It was reported that the problem of continuous cropping obstacles of P. cablin was very severe, which affected the yield, quality and efficacy of this herb medicine. Therefore, it is necessary to study the continuous cropping obstacles of this plant. This project will study the plant allelochemicals extracted from rhizosphere soil using Pogostemon cablin (Blanco) Benth. cv. Hainanensis which will be pot-cultured and simulated the continuous cropping obstacles as the experimental materials. The contents and compositions of the plant allelochemicals will be measured by GC-MS method. By extracting the total DNA of rhizosphere microbes, the changes of rhizosphere microbes from continuous cropping obstacles in P. cablin will be analyzed by using the t-RFLP maps and microorganism training methods.These expression differences of specific proteins from rhizosphere soil will be discussed by the proteomics method, and these interacted proteins from candidate key proteins will be confirmed through the MALDI-TOF/MS method and function display. In summary, the rhizosphere biological characters and molecular ecology mechanism of rhizosphere soil will be systematically studied at the plant allelochemicals, rhizosphere microbes and specific protein levels, and this will provide the theoretical basis for the effective regulations on continuous cropping obstacles of P. cablin and conquering the difficulties of continuous cropping obstacles of other medicinal plants.

广藿香是我国常用芳香化湿类中药之一，具有芳香化浊、开胃止呕、发表解暑功效。连作后的广藿香药材产量和品质明显下降，因而有必要对其连作障碍的成因和作用机制展开研究。本项目对海南广藿香进行盆栽连作试验，从其根际土壤中提取化感作用物质，去除多糖等干扰物，采用 GC-MS 等法分析测试化感物质的成分与含量；提取连作根际土壤微生物总DNA，运用t-RFLP技术分析广藿香根际土壤的t-RFLP图谱，结合微生物培养法，探讨广藿香连作根际土壤微生物的变化趋势；采用差异蛋白质组学方法分析广藿香根际土壤特异蛋白差异表达，通过MALDI-TOF/MS等法鉴定及功能展示，探明广藿香连作候选关键蛋白的互作蛋白。综合化感物质、根际微生物和土壤差异蛋白的研究，探讨广藿香连作障碍的根际生物学过程与分子生态学机制，为实现广藿香连作障碍的有效调控，攻克我国中药材栽培的连作障碍难题奠定理论基础。

广藿香是我国常用芳香化湿类中药之一，具有芳香化浊、开胃止呕、发表解暑功效。连作后的广藿香药材产量和品质明显下降，因而有必要对其连作障碍的成因和作用机制展开研究。本项目对海南广藿香进行盆栽连作试验，从其根际土壤中提取化感作用物质，去除多糖等干扰物，采用 GC-MS 等法分析测试化感物质的成分与含量；提取连作根际土壤微生物总DNA，运用t-RFLP技术分析广藿香根际土壤的t-RFLP图谱，结合微生物培养法，探讨广藿香连作根际土壤微生物的变化趋势；采用差异蛋白质组学方法分析广藿香根际土壤特异蛋白差异表达，通过MALDI-TOF/MS等法鉴定及功能展示，探明广藿香连作候选关键蛋白的互作蛋白。综合化感物质、根际微生物和土壤差异蛋白的研究，探讨广藿香连作障碍的根际生物学过程与分子生态学机制，为实现广藿香连作障碍的有效调控，攻克我国中药材栽培的连作障碍难题奠定理论基础。