扫描电化学显微光谱成像系统的研制与应用

Interface, such as solid / liquid, liquid / liquid, liquid / gas, or three-phase interface, is the most active place for substance interaction. It still remain a challenge to develop new method and technique with highly sensitivity, multi-function and high specificity to study the reaction occurring in the interface in life science and analytical chemistry. Fortunately, the new technique combined by electrochemistry and spectroscopy provide a new pathway for this. This project intends to combine electrochemical techniques, microscopic imaging technology and spectral imaging technology to establish a new approach. The spectral imaging system for scanning electrochemical microscopy can be then designed and developed. The system includes scanning electrochemical microscopy (SECM), spectral microscopy amplification systems, optic fiber converters and spectral imaging system. It will be able to collect date and image related to light, electrochemistry and spectra, respectively, to establish the time-resolved and space-resolved spectral analysis method based on scanning electrochemical microscopy. The instrument is a high integration of SECM and spectroscopy, which can be used to detect information of spectra and electrochemistry from interface reaction, simultaneously. The corresponding process, mechanism and theory will finally be established using this system.

界面（固/液、液/液、液/气，或者三相界面等）是物质相互作用最为活跃的场所，开展高灵敏、多方面、高特异的界面研究新方法和新技术是极具挑战性的生命科学和分析化学研究的前沿，电化学与光谱学的联用提供了一种全新手段，本项目拟将电化学技术、显微成像技术、微区光谱成像技术有机的结合起来，建立一种全新的方法，设计和研制扫描电化学显微光谱成像系统，系统包括：扫描电化学显微镜（SECM），光谱显微放大系统，光纤转换器以及光谱成像系统，系统将可获取光、电化学图像和光谱数据，分别建立时间分辨、空间分辨的扫描电化学显微光谱分析方法，该仪器将SECM和光谱学高度集成为一体，能完成界面反应中光谱信息和电化学信息的同步、高通量、连续测定，探索这些过程的机制，初步建立相关理论。

扫描电化学显微技术是目前最佳的界面电子过程研究方法，但面临无法对光诱导电子转移进行便捷研究的难题，本项目将扫描电化学显微成像与光谱系统有机地集成，统一设计，首次提出了扫描电化学显微镜光谱成像系统，完成了可同步采集光反应中电化学信号和光谱信号的仪器，建立了模拟光合电子转移、光催化纳米膜光电化学性能、传感和识别界面三种适合样机研究的模型体系，进行了如下研究内容：①进行了仪器周边部件-电解池、基底、探针电极、转换光路等的设计加工；研究了模拟光合膜、光敏层、识别界面的修饰及其基于光电子转移的理论研究②利用仪器，对一系列模拟生物膜的异相电子转移速率，各组分间的光电子的传递协同关系进行了研究 ③在该系统的支持下，进行了以光催化纳米膜为代表的光敏膜光电流研究，④进行了纳米酶等的制备及其微区扫描成像研究，同时研究了其界面电子转移和光诱电子转移的动力学；研究了识别界面多步骤电子转移过程，提出相关界面过程动力学理论。