类精神分裂症感觉门控障碍大鼠神经解剖及神经化学

Inhibition of irrelevant and unimportant stimuli is a vital component of all information-processing stages, which is called sensory gating. A large amount of evidences showed that information-processing dysfunctions existed in schizophrenia patients. Structural changes and physiological dysfunctions are biological fundamentals of schizophrenia. Early environmental changes may play important role in neurogenesis of brain. The current studies aimed to investigate the effects of early environmental factors and stress on the formation of sensory gating disturbance. We observed the Nissel, MAP2, Synaptophysin, GC receptors, DA2 receptors, NADPH, TH, ChAT and BDNF stainings in isolate reared SD rats after weaning compared with that in group reared animals by using histochemical, immunohistochemical and in situ hybridization methods. FosB expression was also observed in both groups after chronic pain stimulation. The results showed (1) there had no volume and cell architecture changes in hippocampus between two groups; GC receptors and NADPH positive neurons in hippocampus, ChAT positive interneurons in nucleus accumbence, MAP2 staining in striatum reduced in isolate reared animals compared with that in group reared animals, but TH positive cells in substantia nigra stained deeply and BDNF staining in striatum especially in the marginal division increased in isolate reared rats;(2) no above significance changes were seen in isolate reared and group reared adult animals;(3)FosB positive staining increased in dentate gyrus of hippocampus in isolate reared rats after weaning than that in group. It is implicated that early environment changes has an important effects on the formation of sensory gating dysfunction. As a key structure of sensory gating, hippocampus may have some morphological pathological changes and is vulnerable to stress.

以行为试验所肯定的类精神分裂症感觉门控障碍大鼠模型为研究对象，通过对脑形态学及神经介质改变等的研究，为门控障碍学说提供神经生物学基础的支持，探究早期外界环境因素、应激因素等在精神分裂症发病机制中的作用。此项研究将为精神分裂症的预防及抗精神药物研究提供较充实的理论基础。