微纳尺度电驱动富集稳定性机理与控制方法研究

The sample transport “control-valve effect” restricting the enrichment stability in the process of the micro-nanoscale electrokinetic enrichment is taken as the research object. The multi-disciplinary theory and method, such as continuum mechanics, molecular dynamics and data mining and so, are employed to analysis the influence rules of the factors including the confined micro-nanostructure with the multi-channel gradient, flow resistance structure in the micro-nanofluidic channel, fluid-flow characteristics on the multi-fields potential energy distributions at the micro-nanointerface, and then establish the impacting mechanism model of the potential energy in the flow, ionic and electric fields on the electrokinetic enrichment stability, through considering synthetically with the micro-nanocombinations and coupling relations between the micro-nanostructural and micro-nanointerfacial parameters. According to the experimental test data and theoretical analysis, firstly, the optimal mcrio-nano structure, micro-nano combination, and characteristic parameter of fluid flow are obtained, secondly, the influence principles of the crucial physical parameters and the formation mechanism of the sample transport “control-valve effect” in a nanochannel are mastered, finally, the control methods of the sample transport in the process of the electrokinetic nanofluid enrichment are presented to provide the technique support to the application of the electrokinetic nanofluid enrichment in the micro-nanofluidic chip, such as, DNA detection, immunoassay and seawater purification and so on.

以微纳尺度电驱动富集过程中制约富集稳定性样品输运“控制阀效应”为研究对象，应用连续介质力学、分子动力学以及数据挖掘等多学科理论与方法，综合考虑微纳结合方式、微纳结构及界面参数之间的耦合关系，分析多梯度微纳限域结构、微纳通道内流阻结构、流体流动特性等因素对微纳界面处耦合多场势能分布的影响规律，建立电场、离子场和流场等多场势能对电动富集稳定性影响机理的理论模型。根据实验测试数据和理论分析，获得优化的微纳结构及结合方式和流体流动特性参数，掌握纳米通道中样品输运“控制阀效应”的形成机理及关键物性参数的影响规律，提出电动纳流体富集过程中样品输运的控制方法，为微纳流控芯片中的电动纳流体富集在DNA测试、免疫分析、海水纯化等领域的应用提供技术支撑。

本项目以微纳尺度电驱动富集过程中制约富集稳定性样品输运“控制阀效应”为研究对象，运用连续介质理论综合考虑了微纳结合方式、微纳结构及界面参数之间的耦合关系，分析了微纳限域结构、外加电压、流体流动特性等因素对微纳界面处电场、速度场、浓度场分布的影响规律。根据实验测试数据和理论分析，获得了优化的微纳结构及结合方式和流体流动特性参数，掌握了纳米通道中样品输运“控制阀效应”的形成机理及关键物性参数的影响规律，提出了电动纳流体富集过程中样品输运的控制方法，有望为DNA测试、免疫分析、海水纯化等领域的应用提供技术支撑。