miR156及其靶基因SPL参与花器官形态建成的分子机理研究

The pattern formation in plant is cooperatively regulated by spatial and temporal cues. It has been shown that miR156-mediated age pathway plays diverse roles in plant developmental processes, such as floral induction, trichome formation, leaf initiation rate and anthocyanin biosynthesis. Recently, it has been shown that a group of flower-promoting MADS-box genes contributes to floral patterning. However, whether age pathway participates in floral patterning is still unknown. Here we propose a project to investigate the role of miR156 and its target SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) in floral patterning by multiple approaches such as genetics, reverse genetics and comparative developmental biology. This project will help us to understand the biological function of SPL and the mechanism of spatial and temporal development of plants.

植物的生长发育具有时序性。miR156介导的年龄途径在植物发育过程中扮演了重要角色，参与了植物成花诱导、表皮毛形成、叶起始速率和花色素苷代谢等生物学过程。最新的研究表明，一些成花诱导MADS-box类基因除了调控植物开花时间外，还可以通过与ABCE基因的相互作用，参与花器官形态建成。但是年龄途径在花形态建成中的作用及相关分子机理至今仍不清楚。本课题拟通过遗传学、反向遗传学和比较发育生物学等方法研究miR156及其靶基因SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL)调控花器官形态建成的分子机理。课题的研究对我们理解SPL的生物学功能和植物时序性发育的分子机理有着重要理论意义。