水稻温敏失绿突变体冷激应答反应的分子机理研究

The thermo-sensitive rice line 1103s was a spontaneous chlorophyⅡ mutant found in the 8902s population.The green-yellow bands interrupted discolorated transverse bands on the leaves of the mutant line 1103s was induced by cold shock and the change of leaf color coincided with the change of chlorophyll contents. Ultrastructural observation revealed that the chloroplast structure in the yellow part degenerated, i.e., the number of stocks in granum abnormally decreased, thylakoid system was disintegrated and replaced by few single thylakoid, and then the whole structure of some chloroplast became vesicle-like structure with high electronic density. Nonetheless, in the green zone of the green-yellow banded leaf, there was light decreased in the number granum thylakoids, disordered grana arrangement in the chloroplast, and aggregation of osmiophile globule. However, it was found that these structural alternations were reversable in the process of recovery of the yellow part of the leaves into green, and the chloroplast structure renewed normally. It might be concluded that the character of green-yellow bands induced by cold shock was consequent upon a reverse process of degeneration and regenerantion.By a radial labeling with 14C and SDS-PAGE, study on altering of Rubisco activity and protein, amino acid comptanents in leaves of the mutant line 1103s during expressing process of the green-yellow band trail, was induced that the activity was hardly related with the stable molecular of the large subunit (LS) and small subunit (SS)of Rubisco, But the activity of Rubisco was very closely keeping in contact with an especial protein molecule (５６.２kＤ, PI＝４.５）. While the green-yellow band trail was expressed with the protein disappearing, the activity of Rubisco was reduced. While the trail disappeared in the same part of the leaves with this protein appearing, the activity of Rubisco was increased. It might be concluded that the character of green-yellow bands induced by cold shock was consequent upon the activity of Rubisco controlled by the reverse prosess of the protein decomposition and composition. Meanwhile, it was found that the controlling mechanism of metabolism process of amino aid in the leaves of the chloroplast thylakoid structure degeneration was associated with the accumulation of Glu and Asp.

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