基于铽离子掺杂碳点和DNA分子相互作用的时间分辨荧光型纳米信标研究

Molecular probes with specific recognition effects can be used for studying the mechanism of life activities, disease diagnosis and testing work associated with the environment, justice, food safety. The project aims to combine molecular beacon techniques, carbon dots nanomaterials and rare-earth luminescence probe techniques, to study and apply the time-resolved luminescence probes utilizing terbium ion-doped carbon dots (Tb@CDs) in view of two aspects involving preparation of Tb@CDs and biological sensing analysis. In the project, Tb@CDs are synthesized for the first time, we will study the interaction of Tb@CDs and DNA molecules to illustrate the principle of construction of DNA-sensitized Tb@CDs strategy. A multi-function time-resolved luminescence nanobeacon probe will be constructed by integrated with DNA' s specificity recognition properties and Tb@CDs' excellent physicochemical properties. The proposed nanobeacon probe is a versatile molecular beacon-like (MB-like) probe, can overcome the limitations and expand the scope of application of the traditional molecular beacons. The use of time-resolved luminescence detection technology can remove the background luminescence of samples, which is favorable to biochemical analysis through improving the stability, accuracy and specificity of the nanobeacon probe for practical applications.

具有特异性识别作用的分子探针可用于生命活动机理研究、疾病诊断以及与环境、司法、食品安全相关的检测。本项目拟通过将分子信标技术、碳点纳米材料和稀土荧光探针技术相结合，从铽离子掺杂碳点新材料（Tb@CDs）的制备和生物传感分析两个方面开展基于Tb@CDs的时间分辨荧光型纳米信标研究及应用。我们将首次制备Tb@CDs，研究Tb@CDs与DNA相互作用机理，进一步深入阐释DNA敏化Tb@CDs荧光策略构建原则；并将DNA分子的生物识别特异性整合Tb@CDs独特的光学特性，构建多功能时间分辨荧光型纳米信标探针，该纳米信标是一种通用型分子信标类（MB-like）探针，能克服传统分子信标的局限性并拓展了传统分子信标的应用范围，利用时间分辨荧光检测技术，可去除样品中自发背景荧光，提高探针在生化分析实际应用中的稳定性，准确性和特异性。

具有特异性识别作用的分子探针可用于生命活动机理研究、疾病诊断以及与环境、司法、食品安全相关的检测。本项目中，我们通过将分子信标技术、功能纳米材料和稀土荧光探针技术相结合，实现一步法冷凝回流法制备功能碳点和基于稀土铽离子掺杂碳点（Tb@CDs）与DNA的分子信标及逻辑体系；构筑了基于稀土-核苷酸有机配位聚合物材料生化传感分析体系；构筑了基于DNA的荧光分子信标分析体系；同时针对分子识别及精准检测这一研究领域，发展了一系列比率型荧光分析新方法和基于功能纳米材料的比色传感分析新技术，通过与微渗析活体取样在线分析等技术联用，实现对多种重要靶标的分子识别和精准检测。至今，我们已经完成了项目中的各项任务，在Anal. Chem., Chem. Commun., Biosensors and Bioelectronics等专业期刊上发表SCI收录论文13篇，申请发明专利2项，取得了较好的研究和应用成果。