基于质谱技术分析煎炸老油中脂质氧化化学标记物及其对细胞代谢的研究

There has serious concerns about the deep fried oil (one type of hogwash oils) on the cooking oil markets in China, but there has no standard method for identification of deep fried oil or hogwash oil so far. Moreover, the study of effects on mechanisms of various diseases related deep fried oil and hogwash oil is limited and unclear, too. The aims of the project are: to analyze secondary lipid oxidation compounds in deep fried oil based on development of mass spectrometric methodology; to discover the chemical markers of lipid oxidation for identification of deep fried oil and to initially study the toxicological and metabolic effects of deep fried oil on cells. The roles of the project including: to develop simple, novel and high efficient extraction methods for enrichment of secondary lipid oxidation compounds, such as aldehydes, ketones ethers and alcohols; to optimize the fast, accurate and high throughput methodology for target compounds analysis based on gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry; to profiling analyze lipid oxidation compounds and discover internal chemical markers for identifying deep fried oil; to build a biological cell model and analyze the metabolic and/or toxicological effects of deep fried oil using optimized mass spectrometric methods. The project will provide theoretical and experimental results for deep fried oil identification and also demonstrate the metabolic and further toxicological effects in cell level.

煎炸老油（地沟油的一种）流回餐桌导致恶劣的社会影响, 目前没有标准检测方法，如何导致各种疾病的代谢机理也未明确。本项目拟基于质谱学方法轮廓分析煎炸老油中脂类氧化中的二级氧化产物，筛选化学标记物以鉴别和鉴定地沟油，以及煎炸老油及其氧化物在细胞水平上代谢机理研究。本项目核心包括：开发简单、新颖和高效的提取方法，富集煎炸老油中脂类氧化相对稳定的二级氧化产物，如醛类，酮类，醚类和醇类等；建立简单、快速、准确和高通量的气相色谱-质谱和液相色谱-质谱分析方法；成分轮廓分析氧化产物和筛选其内源性脂质氧化化学标记物，用此方法鉴别和鉴定煎炸老油；建立细胞模型，用优化的高通量的质谱学方法来分析病变细胞和正常细胞暴露在煎炸老油环境中代谢产物的变化，也初步探讨毒理机制，评估煎炸老油及其氧化产物对的代谢机理。本项目可为煎炸老油的氧化产物指标提供理论依据、实验数据和鉴别依据，为煎炸老油的代谢机理研究提供初步的信息。

煎炸老油（地沟油的一种）流回餐桌导致恶劣的社会影响, 目前仍然没有标准检测方法，其如何导致各种疾病的代谢机理也未明确。本项目基于质谱分析方法轮廓分析煎炸老油中异常氧化及水解产物，筛选化学标记物以鉴别和鉴定地沟油和煎炸老油，并对煎炸老油中的氧化产物进行毒性机理研究。本项目核心内容包括：开发简单、新颖和高效的检测方法，建立简单、快速、准确和高通量的气相色谱-质谱，液相色谱-质谱及质谱成像等分析方法；成分轮廓分析氧化及水解产物和筛选其内源性化学标记物，用此方法鉴别和鉴定煎炸老油；并利用质谱方法对煎炸老油中脂质氧化产物的毒性机理进行研究。.本项目的主要研究成果如下：.1..建立了气相色谱-质谱联用方法（GC-MS）鉴别新鲜食用油和煎炸老油；该方法基于轮廓分析及多元统计分析技术，发现游离脂肪酸和甘油单酯在煎炸老油和地沟油样品中显著上升；该现象在连续加热新鲜食用油的实验中得到了验证；结果显示甘油单酯可作为鉴别新鲜食用油和煎炸老油及地沟油的化学标志物；.2..建立了基质辅助激光解吸附质谱成像技术（MALDI-MSI）快速检测鉴别新鲜食用油和煎炸老油；该方法通过对油脂的指纹图谱分析，发现了多种可以区分新鲜食用油，煎炸老油及地沟油的特征离子。该方法可实现对多种油脂样品的快速和可视化分析。.3..利用液相-质谱联用技术，对煎炸老油及地沟油中产生的过氧化物毒性机理进行研究。结果发现脂肪酸类过氧化物可与多种核苷类物质生成加合物。该研究为脂肪酸过氧化物的基因毒性提供了理论依据。