不饱和脂肪酸代谢谱用于孕妇全氟化合物暴露的风险评价及预警的可行性研究

Perfluorinated compounds (PFCs) have been recognized as one class of the global and new persistent organic pollutants commonly detected in blood samples of both wildlife and humans. It is very important to find out the biomarkers in the early exposed to PFCs, which is helpful for the health risk assessment and forewarning, and for taking intervention measures for those exposed to PFCs. The project will be performed by using the pregnant woman population as the research objects. After samples of blood from gravida in early and late pregnancy, and of maternal blood and neonatal umbilical cord blood were collected, the concentrations of PFCs in the blood were firstly detected using high performance liquid chromatography-tandem mass spectrometry. The characteristics and rules for pregnant women and fetuses exposed to PFCs were obtained. Then, the effects of PFCs exposure on pregnant outcomes were discussed. Next, unsaturated fatty acids (UFAs) in blood were detected by gas chromatography-mass spectrometry. The effects of PFCs exposure on pregnant outcomes were also discussed. Finally, the relationship between the concentration of PFCs and UFAs metabolism levels in the blood of paired maternal pregnancy-neonate were analyzed using epidemiological methods. The possibility of UFAs used as health risk assessment and early risk forewarning for pregnant women exposed to PFCs was clarified. The study not only systematically clarify the relationship between pregnancy outcomes and maternal exposure to PFCs, but also first proposed that UFAs profiles in blood were used as the biomarkers of the health risk assessment and the early risk forewarning for pregnant woman exposed to PFCs. The study can provided valuable scientific basis for taking intervention measures for who expose to PFCs.

全氟化合物（PFCs）是一种全球性的新型持久性有机污染物，寻找能反映暴露风险的早期生物学标识物对于健康风险评价、预测和及时采取干预措施具有重要意义。本研究以孕妇为研究对象，釆集孕早、晚期、分娩时母体外周静脉血和新生儿脐带血，首先用高效液相色谱串联质法检测血液PFCs水平，获得孕妇及胎儿PFCs暴露特征及规律，探讨PFCs暴露对妊娠结局的影响；其次用气相色谱-质谱法检测血不同不饱和脂肪酸（UFAs）的水平，分析其对妊娠结局的影响；最后用流行病学方法分析UFAs水平与PFCs暴露之间的关系，阐明血液UFAs水平作为孕妇PFCs暴露对子代健康风险评价和预测的可能性。本研究不仅系统阐明我国孕妇PFCs暴露与妊娠结局的关系，而且首次提出用血UFAs谱作为孕妇PFCs暴露的风险评估和子代早期健康风险预警的生物学标志物，为采取相应干预措施提供科学依据。

全氟化合物(perfluorinated compounds, PFCs)是一种全球性的持久性有机污染物，属于典型的潜在内分泌干扰物质，具有发育毒性、免疫毒性、内分泌干扰及潜在的致癌性等多种毒性效应。本研究通过收集了广州市、深圳市和贵港市孕妇血液，应用UPLC-MS/MS技术测定了其中的14种PFCs的浓度水平，通过统计学分析，获得孕妇PFCs暴露特征及规律；进一步用GC-MS技术测定了相应样本中的脂肪酸的含量，用ICP-MS技术测定了21种元素含量水平。应用统计学方法，分析了血液中PFCs暴露水平对脂肪酸代谢水平的影响以及孕妇血液中PFCs暴露水平与元素含量的相互关系。本研究同时应用所建立的UPLC-MS/MS方法分析了珠三角洲地区7个城市0-7岁儿童血液中PFCs的暴露水平和暴露规律。本研究结果提供了我国经济发达城市以及非经济发达城市孕妇PFCs暴露水平及暴露规律提供基础数据，同时也提供了珠三角洲地区0-7岁儿童PFCs暴露水平及暴露规律。初步阐明了PFCs暴露下孕妇脂肪酸的变化规律以及以EPA等不饱和脂肪酸作为PFCs暴露标志物的可能性，为PFCs暴露的健康风险的分析评估及预警提供了新思路。