苜蓿皂苷调节早期断奶仔猪肝脏代谢的关键候选蛋白鉴定及其网络解析

Under early weaning stress, alfalfa saponins how to regulate expression of related proteins in the liver of piglets and these proteins how to cooperate to improve the antioxidant ability and immunity of piglets, as a result, to promote the growth of piglets? Such research has not been reported. The aim of this project is to clarify the mechanism of alfalfa saponins regulating hepatic metabolism in early weaned piglets from the systemic viewpoint, with the differential proteomics strategy. In our study, firstly, we select and identify the key candidate differential proteins related to alfalfa saponins regulating hepatic metabolism in early weaned piglets using PF2D (protein fraction 2 dimension) system and MALDI TOF/TOF mass spectrometry technology. Secondly, we analyze and annotate the function of these key candidate proteins, so as to understand the beneficial effects of alfalfa saponins on weaning piglets from molecular level. The study have important practical significance for improving modern pig breeding production level in our country, as well as, provide new idea and novel approach to further study the effects of alfalfa saponins on early weaning piglets in the future.

在断奶应激条件下，苜蓿皂苷如何调控仔猪肝脏中相关反应蛋白的表达，它们之间如何协同作用来提高仔猪的抗氧化能力和免疫力，促进仔猪的生长？尚未有人开展研究。本项目从系统的观点出发，用差异蛋白质组学的策略来研究、阐明苜蓿皂苷调节早期断奶仔猪肝脏代谢的机制，通过PF2D（二维高效液相色谱蛋白分离系统）-MALDI TOF/TOF质谱技术来寻找苜蓿皂苷调节早期断奶仔猪肝脏代谢的关键候选差异蛋白，从分子水平解答苜蓿皂苷对断奶仔猪的作用效果这一宏观问题。该研究对于提高我国现代养猪业生产水平将具有重要的理论和实践意义，同时为今后深入研究苜蓿皂苷对早期断奶仔猪的作用机制提供了新思路和新途径。

在断奶应激条件下，苜蓿皂苷如何调控仔猪肝脏中相关反应蛋白的表达，它们之间如何协同作用来提高仔猪的抗氧化能力和免疫力，促进仔猪的生长？尚未有人开展研究。本项目从系统的观点出发，用差异蛋白质组学的策略来研究、阐明苜蓿皂苷调节早期断奶仔猪肝脏代谢的机制，通过iTRAQ技术来寻找苜蓿皂苷调节早期断奶仔猪肝脏代谢的关键候选差异蛋白，从分子水平解答苜蓿皂苷对断奶仔猪的作用效果这一宏观问题。本项目仔猪饲养试验结果表明，适量的苜蓿皂苷可以改善仔猪的生长性能和腹泻，提高仔猪的免疫力及抗氧化能力，促进仔猪健康，结合仔猪肝脏差异蛋白组学分析结果，苜蓿皂苷的这些功效可能是通过调控肝脏代谢相关关键蛋白的表达实现的。同时，差异蛋白组学分析结果还显示，苜蓿皂苷降脂、减肥及抗癌的功效也可能与其对肝脏相关蛋白的调控有关。该研究对于提高我国现代养猪业生产水平将具有重要的理论和实践意义，同时为今后深入研究苜蓿皂苷对早期断奶仔猪的作用机制提供了新思路和新途径。