阿拉伯木聚糖与肠道拟杆菌间的相互作用及其对机体代谢的调控机制

Researches on molecular mechanism of biomacromolecule metabolic regulation have been a great hot topic at home and abroad. Metabolism of human body is not only controlled by their genes, but also regulated by the metabolism of intestinal microbiota. It has been reported in Nature and Science that the effects of biomacromolecle on intestinal microbiota and their metabolites have great significance on body metabolism. Researches have shown that polysaccheride biomacromolecules have obvious effects on intestinal bacteroides which is one of the intestinal dominant bacterias. Deep study is needed for specific interaction between polysaccherides and intestinal bacteroides with their metabolic regulation. Preliminary studies have shown that arabinoxylan could improve intestinal health and obviously increase the ratio of intestinal bacteroides to intestinal microbiota. However, the interaction mechanism between arabinoxylan and intestinal bacteroides with their metabolic regulation are not clear. Based on the previous researches, the present work will deeply focus on the interaction mechanism between arabinoxylan and intestinal bacteroides with their metabolic regulation. Molecular biology, genomics, metabonomics will be carried out as the technology support for this project. The objective of this project is to analyze the interaction between arabinoxylan and intestinal bacteroides, and clarify the regulation of important metabolites in different metabolic pathway. This project will study the molecular mechanism of polysaccheride metabolic regulation from the aspect of intestinal microbiota metabolic process. This research is original and academic leading. It can provide theoretical basis and research model for molecular mechanism of biomacromolecule metabolic regulation in vivo.

生物大分子代谢调控的分子机制已成为国内外关注热点。人体代谢不仅受自身基因控制，更受到肠道菌代谢的调控。Nature、Science等期刊最新报道生物大分子与肠道菌的作用及其代谢物对机体代谢至关重要。研究表明多糖类生物大分子能显著影响肠道优势菌之一的拟杆菌，但其与拟杆菌具体相互作用及对机体代谢影响，尚无深入研究。本课题组前期研究发现阿拉伯木聚糖能促进肠道健康和显著上调拟杆菌在菌群中的占比，但不清楚其与肠道拟杆菌具体相互作用及其代谢调控效应。本课题拟在此基础上采用分子生物学、代谢组学等技术体内外系统研究阿拉伯木聚糖与肠道拟杆菌间的相互作用及其对机体代谢的调控机制这一科学问题。解析两者间相互作用及关键代谢物在机体代谢通路中的调控作用，从肠道微生物代谢这一重要生命过程角度探讨多糖代谢调控的分子机制。该研究具有良好的原创性和学术前沿性，可为其他生物大分子体内代谢调控的分子机制提供理论依据和研究模型。

生物大分子代谢调控的分子机制已成为国内外关注热点。人体代谢不仅受自身基因控制，更受到肠道菌代谢的调控。本项目以阿拉伯木聚糖和同型木聚糖分别作为唯一碳源，从健康人体肠道中筛选拟杆菌，并将其进一步进行纯化和富集培养，探究菌株对不同碳源的利用偏好及相关基因的表达差异，结合多糖降解概况及短链脂肪酸的生成探讨阿拉伯木聚糖和同型木聚糖对不同肠道菌的生长影响机制。采用分子生物学、基因组学、代谢组学、生物信息学等各手段从体外和体内水平系统研究多糖与肠道拟杆菌的相互作用及其对机体代谢调控的分子机制。整体取得一系列原创成果，在本学科领域发表论文共10篇，均标注项目资助号。其中，SCI论文8篇（含封面文章1篇），EI论文1篇，中文核心论文1篇）；授权国家专利4件。项目负责人晋升研究员，入选中国科协“青年人才托举工程”（2018-2020年度）人选（江西迄今唯一获选女性），2019年江西省“双千计划”首批培养类项目--科技创新高端人才（青年），2019年南昌大学赣江特聘教授。获2019年度IUFoST（国际食品科技联盟，全球食品科学领域最高级别国际组织）杰出青年科学家奖（全球每1-2年颁发一次，每次只颁发给一位青年科学家，中国迄今唯一获选女性），2019年中国食品科学技术学会—菲仕兰营养学苑未来科学领袖奖，2018年度江西省科技进步一等奖（排名5），2018年度中国食品科学技术学会科技创新奖--技术进步一等奖（排名5）；项目组成员黄晓君、周兴涛晋升副高职称，博士生高鹤入选江西省优秀博士学位论文；培养博士生6名，硕士生8名。