仿生成釉器生长发育微环境构建釉质仿生矿化模型

This project aims at the key scientific issue in the study of biomimetic mineraliztion of enamel through the acellular strategy. There is a lack of basis to bio-mimic the physical-chemical properties in the micro-environment of enamel development. We propose to simulate the physical-chemical properties of extracellular matrix in enamel organ for constructing an enamel biomimetic mineralization model. This project, firstly, analyzes the physical-chemical properties of enamel matrix in enamel organ during the advanced bell stage, such as viscosity, the size and distribution of fibers, different minerals and their content. Secondly, based on our constructed enamel biomimetic mineralization model, the concentration and gel strength of agarose, as well as the minerals and their content will be optimized according to the obtained parameters in enamel organ. Then the natural enamel matrix proteins (EMP) will be added into this model to construct an agarose hydrogel biomimetic mineralization model with EMP. Lastly, based on the mechanism of the interaction of the key proteins (amelogenin and non-amelogenin), the engineered Leucine-rich amelogenin peptide will be used to simulate amelogenin and acidic glycosylated peptide will be used to simulate non-amelogenin. The two peptides will be added into the agarose hydrogel biomimetic mineralization model to construct a simplified agarose hydrogel biomimetic mineralization model with mimicking EMP. This project will provide new theoretical and experimental basis to study the biomimetic mineraliztion of enamel and the regeneration of tooth structure.

项目针对非细胞策略仿生矿化诱导釉质结构再生研究中，对釉质发育微环境理化性质仿生缺少依据关键科学问题，提出模拟成釉器细胞外基质的理化特性，进行釉质仿生矿化模型构建的学术思想。研究首先剖析实验动物牙胚钟状期晚期成釉器釉质基质的粘度，纤维、孔隙大小分布，无机矿物种类、含量等与矿化相关理化参数；其次，以前期构建的琼脂水凝胶釉质仿生矿化模型为基础，复合一定浓度的天然釉质基质蛋白，依据上述参数优化琼脂浓度和凝胶强度、无机矿物种类含量，使之与成釉器理化特性匹配，即构建“天然釉质基质蛋白-琼脂水凝胶仿生矿化模型”；最后，依据釉质发育的关键蛋白-釉原蛋白与非釉原蛋白之间相互作用机理，采用工程化的富亮氨酸釉原蛋白多肽仿生釉原蛋白，酸性糖基化的多肽仿生非釉原蛋白，通过它们与琼脂水凝胶体系复合，构建简化的“类釉质基质蛋白-琼脂水凝胶仿生矿化模型”。本研究将为釉质仿生矿化、牙体组织再生等研究提供新的理论和实验基础。

项目针对非细胞策略仿生矿化诱导釉质结构再生研究中，对釉质发育微环境理化性质仿生缺少依据关键科学问题，提出模拟成釉器细胞外基质的理化特性，进行釉质仿生矿化模型构建的学术思想。研究首先剖析实验动物牙胚钟状期晚期成釉器釉质基质的粘度，纤维、孔隙大小分布，无机矿物种类、含量等与矿化相关理化参数；其次，以前期构建的琼脂水凝胶釉质仿生矿化模型为基础，复合一定浓度的天然釉质基质蛋白，依据上述参数优化琼脂浓度和凝胶强度、无机矿物种类含量，使之与成釉器理化特性匹配，即构建“天然釉质基质蛋白-琼脂水凝胶仿生矿化模型”；最后，依据釉质发育的关键蛋白--釉原蛋白与非釉原蛋白之间相互作用机理，采用工程化的富亮氨酸釉原蛋白多肽仿生釉原蛋白，酸性糖基化的多肽仿生非釉原蛋白，通过它们与琼脂水凝胶体系复合，构建简化的“类釉质基质蛋白-琼脂水凝胶仿生矿化模型”。本研究为釉质仿生矿化、牙体组织再生等研究提供新的理论和实验基础。本项目目前共发表论文8篇，其中SCI收录1篇。待发表SCI论文1篇。