三维超稳纳米复合材料增敏的农兽药残留快速识别与现场检测技术研究

Food safety issue caused by chemical pollutants is a focus topic in China, even the world. Quickly identification of chemical pollutants and development product for rapid detection is a hot spot in research. Rapid detection of organophosphorus pesticides based on enzyme inhibition and fluoroquinolone drugs based on immunochromatographic strip have the problems of insufficient stability and sensitivity for on-site detection. How to quickly identify chemical pollutants and achieve high sensitivity and stability, is a significant issue in pesticide and veterinary drugs residual analysis. We propose to adopt metal-organic frameworks (MOFs) as novel platforms, using high risk pollutants such as organophosphorus pesticide in fruit and vegetable and fluoroquinolone antibiotics in animal products as the target, innovate synthesis and modification methods, introduce functional biomolecules and nanomaterials, design in-situ entrapping and post-modification paths, prepare super-stable three-dimensional porous nano-frameworks for quickly identifying pesticide or veterinary drugs, develop novel platforms for the immobilization of bio-recognition molecules and signal molecules for pesticides/veterinary drugs, fully promote immobilization efficiency, build integrated systems of recognition-signal readout, develop novel biosensing methods and models, sensitively detect organophosphorus pesticide in agro-products. Therefore, this project is proposed to focus on the following issues including (1) the preparation of novel three-dimensional porous ultra-stable MOFs-based bionanocomposite materials through diversified methods for rapid identifying pollutants, (2) MOFs-based bionanocomposites-based integrated systems of biorecognition-signal readout, (3) developing new biosensing methods for sensitive detection of pesticides and veterinary drugs and application of on-site detection.

化学污染物导致的食品安全问题是国内外关注焦点，其中化学污染物现场快速识别及产品开发是研究热点。基于酶抑制法的有机磷农药和基于金胶试纸条的氟喹诺酮类兽药快检技术存在灵敏度和稳定性不够的问题，如何快速识别化学污染物并实现现场高灵敏度、高稳定性快速检测，是食品中农/兽药残留现场快速分析的难点。本项目以果蔬中有机磷农药和畜产品中氟喹诺酮类兽药为研究对象，基于金属-有机框架材料（MOFs），发展农/兽药生物识别分子和信号分子的高效率、高稳定性多功能固定平台，建立新型识别－信号输出集成/放大系统，开发生物传感新方法快速和高灵敏地检测农兽药，具有重要的学术研究意义和应用前景。本项目主要研究：制备农/兽药特异性识别的三维多孔超稳纳米复合材料；基于三维多孔超稳纳米复合材料的富集与增敏作用，构建农/兽药快速识别－信号输出/放大系统；开发用于农产品/食品中农兽药残留快速灵敏检测的生物传感新技术，并用于现场检测。

化学污染物导致的食品安全问题是国内外关注焦点，其中化学污染物现场快速识别及产品开发是研究热点。基于酶抑制法的有机磷农药和基于金胶试纸条的氟喹诺酮类兽药快检技术存在灵敏度和稳定性不够的问题，如何快速识别化学污染物并实现现场高灵敏度、高稳定性快速检测，是食品中农/兽药残留现场快速分析的难点，因此开发生物传感新方法快速和高灵敏地检测农兽药，具有重要的学术研究意义和应用前景。在本项目资助下，开展了三维超稳材料研制及形貌、性能表征，用于高效固定生物分子及提高灵敏度研究，基于三维多孔超稳纳米复合材料的富集与增敏作用，主要构建农兽药快速识别－信号输出/放大系统，开发用于食用农产品中农兽残留快速灵敏检测的生物传感新技术，并用于现场检测。主要取得了如下进展：（1）超稳材料用于高效固定生物分子及提高灵敏度；（2）多孔金属有机骨架化合物PCN 222材料用于提高有机磷农药快速检测技术灵敏度；（3）基于自发荧光金属-有机框架材料构建了超高灵敏农药快检传感技术；（4）开发了基于竞争法现场快速检测啶虫脒的免疫电化学传感器；（5）研制了对孔雀石绿具有强吸附性能的超负载MOFs膜，为药物富集和检测分析提供了新途径。本项目开发的荧光金属-有机框架纳米材料对有机磷农药的检测灵敏度较国家标准方法（GB5009.199）提高14000倍（甲基对硫磷、对硫磷为例），共计发表9篇高质量的SCI论文，以第一发明人授权国家发明专利6件，另外申报国家发明专利3件，参与制定农药残留快速检测产品评价标准1项，辅助培养研究生5人，其中毕业3人。在论文成果和研究生培养方面均达到预期目标。