CD4+CD25+FoxP3+调节性T细胞在糖尿病视网膜病变中的作用机制研究

Diabetic retinopathy (DR) is the major blinding eye disease. Extensive research and our preliminary work have confirmed: effector t-cell-mediated immunologic injury played an important role in the pathology of DR. CD4+CD25+FOXP3+ regulatory t cells (Treg) is the most important inhibitor of effector of t-cell immune cells. But its mechanism of action is still unknown in the DR. So we propose: Treg cells decrease in number and reduce in vivo functions in DR patients, which may play a key role in the development of DR pathology. First we are planning to exam the distribution and function pattern of the Treg cell subsets in the DR rat model, and then we are planning to block the Treg cells by the specefic antibody to identify the specific functional role in glaucoma.At last we will try to transfer the Treg cell subset back to the rat At last we are planning to block the pathologic T helper cell or transfer the protective Treg cell back the wild type glaucoma mice model. We hope we can further improve the DR pathology theory and raise the new therapeutic target of the clinical treatment. .

糖尿病视网膜病变（DR）是严重的致盲性眼病。大量研究与我们的前期工作均证实：效应性T细胞介导的免疫损伤在DR的病理中起到了重要作用。CD4+CD25+ FOXP3+调节性T细胞（Treg）是机体内最主要的免疫调节细胞，在多种血管性疾病和炎性疾病中起到了关键的促修复作用。但DR患者体内Treg细胞数量减少且功能降低。由此我们提出：Treg的功能活化状态可能在DR的病理过程中起到了关键作用。本课题拟首先全面检测Treg细胞在高血糖大鼠模型中的分化、分布及功能状态。其次通过使用特异性抗体清除Treg细胞，以及过继回输Treg细胞等手段，干预T细胞亚群的失衡机制，从而探讨Treg细胞在DR中的作用机制。本课题首次在细胞、蛋白及基因水平探讨DR的免疫病理，期望能进一步完善DR的病理损伤机制，并为临床DR的防治提供新思路。

糖尿病视网膜病变（DR）是严重的致盲性眼病。课题组在国家自然科学基金的资助下证实了：效应性T细胞介导的免疫损伤在DR的病理中起到了重要作用。CD4+CD25+ FOXP3+调节性T细胞（Treg）是机体内最主要的免疫调节细胞，在多种血管性疾病和炎性疾病中起到了关键的促修复作用。但DR患者体内Treg细胞数量减少且功能降低。我们提出并且证实了，Treg的功能活化状态在DR的病理过程中起到了关键作用。本课题全面检测了Treg细胞在高血糖大鼠模型中的分化、分布及功能状态。其次通过使用特异性抗体清除Treg细胞，以及过继回输Treg细胞等手段，干预T细胞亚群的失衡机制，从而验证了Treg细胞在DR中的作用机制。本课题首次在细胞、蛋白及基因水平探讨DR的免疫病理，为进一步完善DR的病理损伤机制，并为临床DR的防治提供了新思路。