中国特有属蜡梅属植物谱系地理学研究

The Quaternary glacial climate oscillation, making the most of the species distribution changed, have affectted the population genetic structure of the species. Therefore, the study of the genetic variation of species distribution patterns (genetic structure) can be traced back to the history of the geographic distribution of modern plant populations. With the help of the field sampling and molecular phylogeographic methods, the project intends to study three sets of DNA sequences (cpDNA, mtDNA, nDNA) of genetic variation of natural populations Chimonanthus, an endemic shrub genus distribution subtropical regions in the south of China Qinling. According to coalescent theory and other methods of population genetics calculation and simulation, we try to rebulid the evolutionary history of the species lineages and identify its glacial refugia and the expansion route after post-glacial. Results of Chinese subtropical forest species biogeography responsing mode during Quaternary climate change will provide a typical case，and enrich the data bank of other parts of the plant or animal phylogeography.

第四纪冰期的气候变迁，导致多数物种的分布区发生了改变，也进一步影响了物种的种群遗传结构。因此，对物种遗传变异分布模式（遗传结构）的研究可以追述现代植物种群的地理分布历史。本项目拟以中国秦岭以南亚热带地区分布的特有灌木蜡梅属植物为研究对象，利用野外调查取样和分子谱系地理学的方法研究该树种天然群体在单亲遗传叶绿体和线粒体基因以及双亲遗传核DNA三套基因组上的DNA序列遗传变异，确定它们在种群内、种群间和整个地理分布范围上的现代分布格局；利用溯祖理论等方法对群体遗传学数据进行计算和模拟，构建该属物种谱系进化历史，鉴定其冰期避难所和冰期后回迁路线。研究结果对于理解中国亚热带地区森林物种响应第四纪气候变迁的生物地理学模式将提供一个典型案例，也将对其他地区植物或者动物谱系地理学研究具有借鉴意义。