大豆抗大豆花叶病毒病主效QTL的精细定位与候选基因发掘

Soybean Mosaic Virus(SMV) was the main disease and strain SC3 was one of the most widespread strain in China, especially in Huanghuaihai soybean producing areas. Based on analysis of resistance inheritance and QTL mapping to SMV strain SC3 in soybean using recombinant inbred lines by Zhongdou29 cross Zhongdou32, a regional high-density linkage map was to construct by adding more molecular markers to the mapped QTL region and a near-isogenic lines about the target QTL region(QTL-NIL) was to be created to fine map the major resistance QTL to strain SC3 within a 300kb or 0.5cM region.The QTL-NIL parents were inoculated by strain SC3 to analyse expression difference using quantitative reverse transcription polymerase chain reaction(QRT-PCR),in order to find the candidate genes about the major QTL and to confirm the resistance gene finally by sequencing analysis.In a word,the research could be used in marker assisted selection in resistance breeding and had important theoretical and practical significance on improving the level of resistance breeding.

大豆花叶病毒（SMV）是我国最主要的大豆病害之一，其中SC3株系是我国，特别是黄淮海大豆产区最主要的流行株系。本项目旨在利用中豆29×中豆32重组自交系群体（RIL）完成对该株系抗性遗传分析和QTL初步定位的基础上，通过构建QTL-NIL群体和目标区域高密度遗传图谱，不断开发新的标记，将大豆抗SMV SC3株系的主效QTL精细定位在300 kb（0.5 cM）的区间内。利用QRT-PCR技术分析QTL-NIL群体亲本接种SC3后不同时期表达量的变化以初步发掘出主效QTL的候选基因，并通过测序和比较分析进一步确定重要候选基因。总之，该研究结果可为分子标记辅助选择抗病育种提供依据，对提高我国大豆抗病育种水平有着重要的理论和现实意义。

大豆花叶病毒（SMV）是我国最主要的大豆病害之一，其中SC3株系是我国，特别是黄淮海大豆产区最主要的流行株系。本项利用近等基因系群体，完成了对该株系抗性遗传分析，构建了QTL-NIL群体和目标区域高密度遗传图谱，通过标记加密，将大豆抗SMV SC3株系的主效QTL精细定位在142kb的区间内。利用QRT-PCR技术分析QTL-NIL群体亲本接种SC3后不同时期表达量的变化，初步确定了候选基因（Glyma13g38950）。该研究结果为分子标记辅助选择抗病育种提供依据，对提高我国大豆抗病育种水平有着重要的理论和现实意义。