用于痕量草甘膦检测的荧光探针设计合成及应用研究

Glyphosate as a widely used herbicide, which poses a threat to human health through the residue in crops and the environment, therefore, more and more attention is being focused on the detection of glyphosate in crops and the environment. In this project, the fluorescence probe detection is applied to the analysis of trace glyphosate in crops. This project is based on the structure of glyphosate, novel fluorescent probes are designed and synthesized. And then, recognition mechanisms are explored, effects of amino and carboxyl groups on the identification process are investigated, mechanisms of corresponding fluorescence effect are clarified. For the above, theoretical basis for the fluorescence detection of glyphosate is provided. The various influence factors such as solvent, pH and different manions are investigated in the detection process, responsivity and detection limit of the probe molecules are explored, the optimal test conditions are determined. The imaging performance of probe molecular are investigated in cells, and the fluorescence probe detection method to detect the residue of glyphosate in the fruits of transgenic crops are applied. The research mentioned above provide a brand new, rapid and efficient method to detect the residue of glyphosate for the agricultural production and food industry.

草甘膦作为一种广泛使用的除草剂，其残留在环境、农作物中会危害人体健康，因此，越来越多的注意力集中在农作物和环境中草甘膦的检测上。本项目提出将荧光探针检测法应用于农作物中痕量草甘膦的分析检测。本项目拟从草甘膦结构出发，设计并合成结构新颖的荧光探针分子，探究探针分子对草甘膦的识别机理，考察草甘膦结构中氨基、羧基等基团对识别过程的影响，阐明相应荧光效应产生机制，拟为草甘膦的荧光检测提供理论基础；考察检测过程中溶剂、pH、不同阴离子等多种影响因素，探究探针分子对草甘膦的响应度及检测极限，确定最佳测试条件；考察探针分子细胞成像性能，并将荧光探针检测法用于转基因作物果实中残留草甘膦检测，拟为农业生产及食品行业中草甘膦的痕量检测提供一种全新、快速、高效的检测方法。

随着草甘膦在除草领域中的广泛应用，残留在环境及农产品中的草甘膦最终会通过食物链传递进入生物体内产生多种危害，如可引发肺癌、血管瘤等疾病的发生，还会引发肝肾毒性、生殖毒性等危害。因此，对环境及农产品中草甘膦残留水平的监测具有重要意义。本项目的主要研究内容是将荧光传感检测法用于草甘膦的痕量检测，设计合成铜离子或锌离子络合型探针，利用草甘膦结构中膦酸基、羧基及仲胺基等基团与铜离子或锌离子较强的络合能力，通过配体置换夺取探针-金属络合物中的金属离子，产生荧光信号变化实现对草甘膦的特异性定性、定量检测，提供一种便捷的草甘膦检测方法；阐明测试溶剂、pH、响应时间、金属离子、阴离子及有机磷农药等因素对检测过程的影响，建立高效、特异、抗干扰及可视化的草甘膦荧光检测法；阐明荧光传感体系对草甘膦的检测极限，实现草甘膦的高灵敏检测；阐明荧光传感体系对草甘膦的检测机理，为草甘膦的荧光检测提供理论依据；本项目设计的荧光传感体系可实现水体、土壤、大豆及玉米等中痕量草甘膦检测，为环境、农业及食品行业中草甘膦检测提供一种高效、便捷的检测方法。