苦参碱诱导白血病细胞凋亡分子机理的研究

The induction of tumor cell apoptosis in one of important methods in treatment of.tumors. For the moment, the drugs used for inducing leukemia cell apoptosis are.mainly ones containing heavy metal with severe toxicity. In this study, matrine, analkaloid with definite anticancer effect but no obvious side effect, was used for.inducing different kinds of leukemia cell lines and the genes closely related to.apoptosis modulation, were analyzed by several molecular methods such as DNA.chips array etc. Thereafter, some direct evidence will be provided for matrine in itsclinical use.

诱导肿瘤细胞凋亡是治疗肿瘤的重要手段之一。目前临床应用的诱导白血病细胞凋亡的药物主要是重金属类制剂，毒副作用大。本课题拟用有抗肿瘤作用且无明显毒副作用的苦参碱作用于不同类型白血病细胞系及所制备的荷瘤裸鼠模型，研究其诱导肿瘤细胞凋亡及其主要调控基因P53、bcl-2等改变，为苦参碱诱导白血病细胞凋亡的临床应用提供理论依据。