"万年生"野生稻内生菌多样性及分子系统进化研究

The wild rice grown in the different natural ecological environment (for example the wild rice lives ten thousand years in Guangxi) is a very important and rar germplasm resources. With the expanding and the aggravating of human activity, these rarely wild rice will be dissapeared, together lost with the ancient endophytic diazotrophs evolutionally. To conserve the maximum diversity of the anciently endophytic bacteria in the wild rice(wild rice will conserve in the 'Wild Rice Core Collection Nursery of South China Agricultural University' with original soil), the collection of endophytic nitrogen-fixing bacteria isolated from the wild rice grown in Guangxi provice are going on. We try to clarify the diversity of endophytic bacteria colonized in wild rice, the dominant species and their distribution in different plant tissue by the characterization of physiological and biochemical properties, determination of resistance, tolerance analysis, molecular genetics and phylogentic analysis of their taxonomic status and evolutionary relationships systematically. We also try to show the diversity of endophytic bacteria in rice germplasm resources, to develop the diversity function of endophytic bacteria ( such as biological active substances, antibacterial substances, nitrogen fixation, growth promotion, disease resistance, promoting plant repair), and to reveal the relevance among the diversity of endophytic diazotrophs, the host plants and ecologically geographic distribution. The screening of the suitable combination of the dominant endophytic bacteria markered with rfp or gfp gene will be inoculated with the rice plants to trace the location and distribution in the rice tissue, and the promotion growth of rice plants.

原始生态"万年生"野生稻是一种很重要和稀缺野生稻种质资源，随着人类生产活动范围扩大和加剧，这些重要种质资源在村民的生产活动中可能很快消失，尤其是包含了进化古老的内生菌（包括固氮菌）株资源也随之消失。项目拟对广西不同生态的野生稻资源及内生菌资源进行收集和保存（带原始生态土壤保存于华南农业大学野生稻核心收集圃）。通过生理生化测定、抗性测定、耐受性分析、遗传型特征和分子发育分析，系统地确定它们的分类地位和进化关系。建立多样性的稻属内生菌种质资源库，挖掘多样性内生菌的功能（如生物活性物质、抗菌物质、固氮作用、促生作用、抗病虫害、促植物修复等）。阐明稻属植物中内生固氮菌资源多样性、优势类群及其在植物组织中的分布状况；揭示内生菌多样性与宿主植物、生态地理分布相关性；发现合适匹配的优势内生菌组合，进行荧光蛋白基因rfp或gfp标记，接菌水稻后，观察、跟踪在水稻组织中的存在和分布及促进水稻等植物生长情况

原始生态"万年生"野生稻是一种很重要和稀缺野生稻种质资源，随着人类生产活动范围扩大和加剧，这些重要种质资源在村民的生产活动中可能很快消失，尤其是包含了进化古老的内生菌（包括固氮菌）株资源也随之消失。项目对广西不同生态的野生稻资源及内生菌资源进行收集和保存（带原始生态土壤保存于华南农业大学野生稻核心收集圃）。通过生理生化测定、抗性测定、耐受性分析、遗传型特征和分子发育分析，系统地确定它们的分类地位和进化关系。建立多样性的稻属内生菌种质资源库，挖掘多样性内生菌的功能（如生物活性物质、抗菌物质、固氮作用、促生作用、抗病虫害、促植物修复等）。阐明稻属植物中内生固氮菌资源多样性、优势类群及其在植物组织中的分布状况；揭示内生菌多样性与宿主植物、生态地理分布相关性；发现合适匹配的优势内生菌组合，进行荧光蛋白基因rfp或gfp标记，接菌水稻后，观察、跟踪在水稻组织中的存在和分布及促进水稻等植物生长情况。