马铃薯抗旱相关基因StHSP的克隆及功能分析

Potato is the third most important staple food crop in terms of consumption in the world. Potato is sensitive to water deficit because of its shallow root system distribution. The yield and quality of potato were seriously affected by drought stress. It is of great significance to effectively screen out key regulate drought-resistant genes and to reveal regulatory network of stress response. It will be good for further researching molecular drought response mechanism. In previous studies, potato varieties with different drought resistance were used as experimental material, we used real - time PCR technology to analyze the different upregulated protein genes under drought stress. We chose StHSP as candidate genes because it was highly induced in all varieties under drought stress, it could be one of the key genes regulated by potato drought resistance. In this research, StHSP gene will be cloned, and the phylogenetic tree of purpose gene will be established. Genetic diversity will be analysised from gene sequence, and the gene characteristics of space and time expression will be studied in the level of gene expression. At the same time we research gene function by over-express and RNAi technologies. In all, the whole study is trying to elucidate the drought resistance mechanisms, and to provide theoretical evidences for the studies of crop drought resistance and breeding.

马铃薯是世界第三大粮食作物，由于其根系分布浅对水分亏缺十分敏感，干旱胁迫严重影响其产量和品质。发掘抗旱关键调控基因，解析胁迫响应分子调控网络，对深入研究马铃薯干旱胁迫应答的分子机理意义重大。利用real-time PCR技术，检测干旱诱导上调表达蛋白基因，StHSP基因在抗旱性不同的马铃薯品种中均显著上调，其有可能是马铃薯抗旱调控的关键基因之一。因此，本研究拟对马铃薯StHSP基因进行全长克隆、构建系统发育树；并从基因序列出发分析基因的多样性、从基因的表达水平上研究基因的时空表达特性，最后对基因进行过表达和RNAi明确基因功能，进一步验证揭示StHSP基因抗旱的分子机制，明确马铃薯抗旱机制，为提高作物抗旱性及抗旱品种选育提供理论依据。