拟南芥种子休眠QTL位点DOG3和DOG19的克隆和功能研究

Seed dormancy is a pivotal ecological and agricultural trait which directly determine the timing of seed germination and affect crop yield and food quality in agriculture. However, little is known about the specific molecular mechanisms underlying this trait. In the proposal, based on the identification of DOG3 and DOG19 which are two major QTL loci of seed dormancy, we will further investigate the function of DOG3 and DOG19 using several approaches such as molecular genetics, omics and biochemistry. The cloning and function research of DOG3 and DOG19 would improve our knowledge about seed dormancy, and also can supply good candidate genes for molecular breeding of crops.

种子休眠是植物由生殖生长过渡到营养生长的重要转变期，是关乎植物生存和作物产量及品质的重要性状，因此种子休眠的分子调控机制研究不仅具有重要的理论价值还可服务于农业生产。当前对种子休眠调控特异性的分子网络了解较少，项目拟以种子休眠调控主效QTL位点DOG3 和 DOG19的克隆鉴定为出发点，结合已有的种子休眠和萌发突变体如aba1,abi3,etr1,ga1等，综合运用生理生化，分子遗传和组学等现代生物技术手段对DOG3和DOG19在植物种子休眠和萌发分子调控中的作用机理进行全面深入的研究，以期丰富植物调控种子休眠的特异性分子网络，为农业生产分子辅助育种提供理论依据和基因资源。

种子休眠是关系植物繁衍和作物高产稳产的重要性状，目前对其调控的分子机理还了解较少。该项目拟克隆拟南芥种子休眠主效QTL 位点DOG3 和 DOG19 、再结合基因功能研究和遗传分析进一步深入了解植物调控种子休眠的分子基础，探讨植物调控种子休眠的特异性分子网络，为人工调控休眠提供理论依据。通过课题的资助，我们明确了DOG3的精确位置，突变体和转基因株系分析发现DOG3可能为非编码RNA，结合遗传和组学的进一步研究发现DOG3可能通过调控mRNA的稳定性调控种子休眠，这是种子休眠领域又一新的调控途径，其具体的分子调控机制还有待进一步深入研究。