基于新型手性整体柱的毛细管电色谱微分离技术在手性兴奋剂中的研究与应用

Stimulant drugs residues is one of the important factors for human health and food safety, especially, the diefference of chirality leads to the different perniciousness. Therefore, it is significant for the separation and ultra trace detection of chiral stimulant drugs residues. Nowadays, many challenges including ultra trace amount of steroid hormones, the method of high sensitivity, high efficient separation technology still exist. For these difficulties, the research intends to establish methods for the effective separation and the high sensitivity detection by a new chiral monolithic column based on cinchona alkaloid. As capillary electrochromatography for the analysis method, it is focusing on the preparation of chiral capillary electrochromatography monolithic column for separation, Multiple enrichment technologies-capillary electrochromatography platform have built to meet the ultra or trace amounts of high sensitive detection of chiral stimulant. The topic plays an important guiding aspects of the chiral stimulant mechanism for human health by the precise qualitative and quantitative study. At the same time, the topic can rich the technology for separation and detection of chiral stimulant and expand to applications of capillary electrochromatography, which has important theoretical value and good prospects.

兴奋剂药物残留是影响人体健康与食品安全重要因素之一，特别是手性差异导致危害性差异较大。因此，对手性兴奋剂药物残留的分离与痕量检测具有重要意义。目前，手性药物痕量分析、高灵敏度检测、高效分离等方面存在诸多难点。针对现有存在困难，本课题开展以金鸡钠生物碱分子为基石制备新型手性整体柱，高效分离，高灵敏度检测方法的研究。以毛细管电色谱方法为基础，重点制备新型手性聚合物整体柱，构建多重富集技术-毛细管电色谱检测平台以满足痕量手性兴奋剂药物残留的高灵敏检测方法。本课题通过精准地定性与定量分析对手性兴奋剂对人体健康危害作用机理等方面的研究具有指导作用。同时，本课题的研究丰富手性兴奋剂的分离与检测技术，拓展毛细管电色谱的应用领域具有重要的理论研究价值和良好应用前景。

本项目基本围绕聚合物整体柱制备及应用、石墨烯材料结合色谱及毛细管电泳、电化学方法在分离分析中应用研究。首先围绕利用L-半胱氨酸（L-Cys）是一种两性离子亲水性化合物，含有在二氧化硅微球上修饰了丰富的氨基和羧基，制备了纳米复合材SiO2@ L-Cys毛细管填充柱，用于富集糖肽，同时，聚合物整体柱与高效毛细管电泳技术联用，建立了2-萘氧乙酸、1-萘乙酸、3-吲哚乙酸、3-吲哚丁酸以及6-苄氨基嘌呤五种植物激素的整体柱微萃取与分离检测方法，获得良好分离与检测效果。其次，在实验研究过程中，利用纳米金粒子富集与毛细管电泳测定水产品中4种氟喹诺酮类药物残留、硝基呋喃类药物残留，还开发纳米颗粒（Au-NPs-Yb）用于检测有机磷农药（OPs），均获得良好检测效果。此外，项目中还开发了石墨烯－二氧化锰分散液于玻碳电极上，然后利用循环伏安法将1 -辛基-3 -甲基咪唑六氟磷酸盐修饰，制得石墨烯－二氧化锰－离子液体修饰电极，研究了修饰电极对尿酸、抗坏血酸和黄嘌呤混合物电化学行为，效果较好。..