基于串行金字塔波前传感方法的受激辐射损耗显微镜像差补偿技术研究

By introducing a depletion beam to inhibit the potential fluorescence, stimulated emission depletion (STED) microscopy is able to achieve a spatial resolution beyond the diffraction limit. However, the system aberrations and optical aberrations introduced by specimens will seriously reduce imaging quality of STED microscopy and limit the imaging depth. Adaptive optics technology plays an important role for the compensation of aberrations. Our project proposes an adaptive optical STED microscopy based on sequential pyramid wavefront sensing method, which is a new method. If sequential pyramid wavefront sensor is applied to adaptive optical STED microscopy, it will be helpful to improve the bandwidth of imaging and reduce the effects of photobleaching. Our project focuses on the two main aspects: .1) The theoretical model and the numerical simulation of sequential pyramid wavefront sensing method, which will provide theoretical basis for establishing the experimental system; .2) The experimental study on adaptive optical 3D scanning STED microscopy with the sequential pyramid wavefront sensor. .The study on adaptive optical STED microscopy based on direct wavefront sensing method has not been proposed, to our best knowledge. We believe that the research on these topics would produce high quality research results.

受激辐射损耗(STED)显微镜通过引入一束损耗光的方式减小有效荧光发光面积，可以实现超衍射极限空间分辨率。但是系统像差以及由实验样本引入的光学像差，将会严重降低STED显微镜成像质量并限制成像深度。自适应光学技术对于补偿光学像差具有重要作用。本项目提出开展基于串行金字塔波前传感方法的自适应光学STED显微镜研究。串行金字塔波前传感方法作为一种新型波前传感方法，应用于自适应光学STED显微镜有助于提高成像带宽，降低光漂白效应影响。本项目主要研究内容包括：. 1)完善串行金字塔波前传感方法相关理论模型并开展数值模拟研究，为实验系统建立提供理论依据；. 2)开展基于串行金字塔波前传感器的自适应光学三维扫描STED显微镜实验研究。. 目前，基于直接波前传感方法的自适应光学STED显微镜研究尚未开展。项目组相信，对以上内容的研究极有可能产生高水平研究成果。

受激辐射损耗显微镜通过引入一束损耗光的方式减小有效荧光发光面积，可以实现超衍射极限空间分辨率。但是系统像差以及由实验样本引入的光学像差，将会严重降低显微镜成像质量并限制成像深度。自适应光学技术对于补偿光学像差具有重要作用。本项目开展了基于串行金字塔波前传感方法的自适应光学显微成像研究。串行金字塔波前传感方法作为一种新型波前传感方法，应用于自适应光学显微镜有助于提高成像带宽，降低光漂白效应影响。本项目主要完成研究内容包括：1)完善串行金字塔波前传感方法相关理论模型并开展数值模拟研究，为实验系统建立提供理论依据； 2)开展了基于串行金字塔波前传感器的自适应光学成像实验研究。串行金字塔自适应光学技术研究开辟了新的思路，并推进我国在该领域的研究达到国际先进水平。