基于高通量测序的细菌sRNA-靶标mRNA相互作用识别研究

Bacterial sRNAs are a class of regulatory RNAs with their lengths varying from 40nt to 500nt. Its main role is to carry out post-transcriptional regulation on the target genes through sRNA-target mRNA interaction, which plays an important role in bacterial virulence, quorum sensing, and sensing the enviromental changes. Therefore, studying sRNA-mRNA interaction is very important. The project reported here intends to invesitgate bacterial sRNA-target mRNA interaction comprehensively using biotechniques such as high throughput sequencing and bioinformatics methods like RNA secondary structure prediction. The project can not only identify the bacterial sRNAs and their target mRNAs simultaneously, which provide experimental support for studying sRNA functions deeply, but also provide data support for developing prediction models for bacterial sRNAs and their target mRNAs. Finally, the project will promote the functional genomics study of bacterial sRNAs, and understanding the activity of bacterial life.

细菌sRNA是细菌中普遍存在的一类长度在40～500个核苷酸的调控RNA，主要通过与靶mRNA结合实现基因表达的转录后调控，进而在细菌毒性、群落感应和对外界环境的反应等方面发挥重要作用，因此，开展细菌sRNA-靶标mRNA相互作用研究具有重要意义。本项目拟综合运用高通量测序等生物技术与RNA二级结构预测等生物信息学方法系统开展细菌sRNA-靶标mRNA相互作用研究，该策略可以同时识别细菌sRNA及其靶标mRNA，不仅为阐明特定条件下细菌sRNA功能提供实验指导，也为开发细菌sRNA及其靶标预测模型提供数据支持，最终促进细菌sRNA功能基因组学研究和人们对细菌生命活动的理解。

在该课题资助下，我们按计划主要开展了以下研究。1，基于高通量测序的RNA-RNA相互作用预测研究，发现体外条件下测定的RNA相互作用不能完全真实反映体内的RNA相互作用；2，基于高通量测序的大肠杆菌中RNA-RNA 相互作用研究，建立了相应实验技术与生物信息学注释分析平台，构建了相应数据库和网络服务器 ；3，构建了细菌sRNA靶标数据库及sRNA功能在线注释系统，以及sRNA-mRNA组合调控预测模型及相应服务器。基本完成预定研究目标。