濒危藏药独一味繁殖策略及对草地退化的响应机制研究

Lamiophlomis rotata is an endangered Tibetan medicine facing the problem of resource shortage. The study of population reproductive characteristics lagged far behind its application. There were bottleneck problems difficult to work out such as protection and breeding of the wild population and artificial breeding of Lamiophlomis rotata. The investigation showed that Lamiophlomis rotata could adapt to plateau grassland of different degradation degree in special regeneration mode. The number of plants in population of Lamiophlomis rotata broke out in one or several age classes. The population regeneration style maybe special. In view of this, the project brought out the hypothesis of “opportunistic reproductive strategy” and try to illustrate the response mechanism of the population regeneration to grassland degradation. Wild investigation and stress experiments will be carried out to get the information of seed production, reproduction cost and physiological indexes in different degraded habitats. And obtain the effect of low temperature-drought stress on the seed germination, seedling growth and physiological and biochemical characters of Lamiophlomis rotata. Then find out the key ecology factor influenced the reproductive ability and seedling establishment by statistical methods such as factor analysis. Analyze the relationship among the key ecological factor, climatic factor and soil degradation. Coupling the climatic flucturation and outbreak of age class. Finally, test the hypothesis and clarify the reproductive strategies of Lamiophlomis rotata in degraded grassland. The study could forecast the development trend of Lamiophlomis rotata population, protect the its resource and promote its industrial development. The study also have theoretical reference value for illustrating life history strategy of perennial plants in plateau.

独一味Lamiophlomis rotata是一级濒危藏药，资源紧缺。其种群繁殖特征研究滞后，野生种群保育、人工种植等瓶颈问题难于突破。调查显示，独一味能适应不同退化程度的高原草地，表现出某个或几个龄级数量的爆发，种群更新模式特殊。鉴于此，本项目提出独一味“机会主义繁殖策略”假说，试图阐明种群更新对草地持续退化的响应机制。野外原位调查与胁迫实验相结合，观测不同退化生境下独一味种子产量、繁殖成本、生理指标；分析低温-干旱胁迫对种子萌发、幼苗生长及生理生化特征的影响。再通过因子分析等手段寻找影响独一味繁殖能力、幼苗定居的关键生态因子。进而分析该因子与气候因子、土壤退化的关系，对气候波动与龄级爆发进行耦合，验证假说，阐明独一味在退化草地的繁殖策略。本研究对于预测独一味种群发展趋势，保护其野生资源，探索适宜的生产发展模式均具有重要意义，亦对于阐明高原多年生宿根植物的生活史对策具有理论参考价值。

独一味Lamiophlomis rotata是一级濒危藏药，资源紧缺。本研究表明，独一味符合集合种群有关特征，体现出一些种群迁徙性，独一味在不同退化草地中多呈现出“铃型种群模型。独一味种群适应了退化生境，通过深长根系，对土壤肥力胁迫、冻融胁迫均有良好的适应性；独一味中度退化草地对独一味胁迫更大，独一味种群往往增加种群密度、降低植株大小，繁殖能力活跃，独一味往往根系分泌物旺盛，SOD、POD、MDA、PRO均与其他退化等级草地独一味有显著差异，且体现出含量积累的增加，这可能与干旱胁迫有一定关系。本研究对于预测独一味种群发展趋势，保护其野生资源，探索适宜的生产发展模式均具有重要意义，亦对于阐明高原多年生宿根植物的生活史对策具有理论参考价值。