具有光调控性质人工跨膜通道的组装及其功能研究

In this project, the photo-regulated artificial transmembrane systems were proposed. Based on the structural properties of pillar[5]arene, the artificial channels were designed by introducing of the side chains incorporated with azobenzene which is sensitive to UV light. The intramolecular hydrogen bonding was designed to induce the channels to self-assembled to form tubular structures. The transmembrane transport of ions, water and amino acids of the channels will be investigated on vesicles and planar lipid bilayer. The UV light will be applied to regulate the transport process by changing the conformation of the channels.

本项目提出了一类具有光调控功能的人工跨膜输送体系。基于柱[5]芳烃的官能团定向特征，通过引入含有偶氮苯基团的侧链，利用分子内氢键驱动组装形成单分子的人工跨膜通道。设计在由磷脂分子构成的囊泡及平面脂双层上开展离子、水和氨基酸的跨膜输送研究，并设计通过光调节通道分子的构象来调控通道分子跨膜输送的效率和选择性，实现可控的跨膜输送。

在本项目的资助下，通过精确结构设计，我们不仅实现了对人工通道跨膜输送开关的可逆光调控，构筑了具有光响应性的人工通道，实现了对K+跨膜输送方向的控制，而且还实现了人工通道对细菌细胞膜的选择性识别和嵌入，达到了高效的抑制细菌生长的目的，同时还展现出很低的细胞毒性，发展了一种构建细胞膜活性抗生素的新策略。发表标注受本项目资助的论文6篇，包括2篇Angew. Chem. Int. Ed.论文，其中有关人工通道选择性识别细菌细胞膜的工作入选Angew. Chem. Int. Ed.当期的VIP论文。通过本项目培养研究生5名。在项目执行期内，项目负责人获得国家杰出青年基金的资助及首届中国化学会超分子化学青年创新学术讲座奖。